This research was supported by Grant RO1MH 49248 from the National Institutes of Mental Health.
Imidazoline Receptor Antisera-Selected cDNA Clone and mRNA Distributiona
Article first published online: 6 FEB 2006
Annals of the New York Academy of Sciences
How to Cite
PILETZ, J. E., JONES, J. C., ZHU, H., BISHARA, O. and ERNSBERGER, P. (1999), Imidazoline Receptor Antisera-Selected cDNA Clone and mRNA Distribution. Annals of the New York Academy of Sciences, 881: 1–7. doi: 10.1111/j.1749-6632.1999.tb09335.x
- Issue published online: 6 FEB 2006
- Article first published online: 6 FEB 2006
ABSTRACT: A novel cDNA, designated Imidazoline Receptor Antisera-Selected cDNA-1 (iras-1), encodes a 167-kD protein. Two of its predicted peptides (42–43 kD) are immunologically consistent with a previously reported 11-imidazoline binding protein. In the present study, two forms of iras mRNA (6.0 and 9.5 kb) were quantified across fresh rat tissues. Highest levels were found in brain (almost exclusively 6.0 kb in size), followed by liver and lung (9.5 ≥ 6.0 kb iras mRNA), kidney (6.0 > 9.5 kb), heart (6.0 kb), spleen (6.0 ≥ 9.5 kb), testes (6.0 > 9.5 kb), and skeletal muscle (6.0 > 9.5 kb). A correlation exists (ρ= 0.71, p= 0.05) between total (6.0 + 9.5 kb) iras mRNA and I1 BMAX values across rat tissues, corrected for housekeeping gene expression. Thus, total iras mRNA appears to be roughly proportional to the density of I1-imidazoline binding sites.