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Imidazoline Receptor Antisera-Selected cDNA Clone and mRNA Distributiona

Authors

  • JOHN E. PILETZ,

    Corresponding author
    1. Department of Nutrition, Case Western Reserve School of Medicine, 10900 Euclid Ave., Cleveland, Ohio 44106–4982, USA
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  • JULIA CAY JONES,

    1. Departments of Psychiatry, Pharmacology, and Physiology, University of Mississippi Medical Center, 2500 North State St., Jackson, Mississippi 39216–4505, USA
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  • HE ZHU,

    1. Departments of Psychiatry, Pharmacology, and Physiology, University of Mississippi Medical Center, 2500 North State St., Jackson, Mississippi 39216–4505, USA
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  • OSAMA BISHARA,

    1. Department of Nutrition, Case Western Reserve School of Medicine, 10900 Euclid Ave., Cleveland, Ohio 44106–4982, USA
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  • PAUL ERNSBERGER

    1. Department of Nutrition, Case Western Reserve School of Medicine, 10900 Euclid Ave., Cleveland, Ohio 44106–4982, USA
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  • a

    This research was supported by Grant RO1MH 49248 from the National Institutes of Mental Health.

jpiletz@psychiatry.umsmed.edu; phone, 601 984–5898; fax, 601 984–5899

Abstract

ABSTRACT: A novel cDNA, designated Imidazoline Receptor Antisera-Selected cDNA-1 (iras-1), encodes a 167-kD protein. Two of its predicted peptides (42–43 kD) are immunologically consistent with a previously reported 11-imidazoline binding protein. In the present study, two forms of iras mRNA (6.0 and 9.5 kb) were quantified across fresh rat tissues. Highest levels were found in brain (almost exclusively 6.0 kb in size), followed by liver and lung (9.5 ≥ 6.0 kb iras mRNA), kidney (6.0 > 9.5 kb), heart (6.0 kb), spleen (6.0 ≥ 9.5 kb), testes (6.0 > 9.5 kb), and skeletal muscle (6.0 > 9.5 kb). A correlation exists (ρ= 0.71, p= 0.05) between total (6.0 + 9.5 kb) iras mRNA and I1 BMAX values across rat tissues, corrected for housekeeping gene expression. Thus, total iras mRNA appears to be roughly proportional to the density of I1-imidazoline binding sites.

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