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The I1-Imidazoline Receptor and Its Cellular Signaling Pathways

Authors

  • PAUL ERNSBERGER

    Corresponding author
    1. Departments of Nutrition, Medicine, Pharmacology, and Neuroscience, Case Western Reserve School of Medicine, Cleveland, Ohio 44106–4906, USA
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Address for correspondence: Paul Ernsberger, PhD, Department of Nutrition, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106–4906, USA. Phone, 216 368–4738; fax, 216 368–4752; e-mail, pre@po.cwru.edu

Abstract

ABSTRACT: Two primary questions are addressed. First, do I1-imidazoline binding sites fulfill all the essential criteria for identification as a true receptor? Second, what are the cellular signaling pathways coupled to this novel receptor? I1-imidazoline binding sites show specificity in binding assays, linkage to physiologic functions, appropriate anatomic, and cellular and subcellular localization. Most important, binding affinities correlate with functional drug responses. I1-imidazoline binding sites meet several additional criteria identified with functional receptors: they show physiologic regulation and endogenous ligands and, most crucially, are coupled to cellular signaling events. A series of studies have identified cellular events triggered by I1-imidazoline receptor occupancy. This receptor is not coupled to conventional pathways downstream of heterotrimeric G-proteins, such as activation or inhibition of adenylyl or guanylyl cyclases, stimulation of inositol phospholipid hydrolysis, or induction of rapid calcium fluxes. The I1-imidazoline receptor is coupled to choline phospholipid hydrolysis, leading to the generation of diacylglyceride, arachidonic acid, and eicosanoids. Additional cellular responses include inhibition of Na+/H+ exchange and induction of genes for catecholamine synthetic enzymes. The signaling pathways linked to the I1-imidazoline receptor are similar to those of the interleukin family, implying that I1-receptors may belong to the family of neurocytokine receptors.

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