In vitro data presented in this paper and were first published in Proceedings of the National Academy of Sciences (July 1998, 95: 8268–8273).
Neuroprotective Antioxidants from Marijuana†
Article first published online: 25 JAN 2006
Annals of the New York Academy of Sciences
How to Cite
HAMPSON, A. J., GRIMALDI, M., LOLIC, M., WINK, D., ROSENTHAL, R. and AXELROD, J. (2000), Neuroprotective Antioxidants from Marijuana. Annals of the New York Academy of Sciences, 899: 274–282. doi: 10.1111/j.1749-6632.2000.tb06193.x
- Issue published online: 25 JAN 2006
- Article first published online: 25 JAN 2006
Abstract: Cannabidiol and other cannabinoids were examined as neuroprotectants in rat cortical neuron cultures exposed to toxic levels of the neurotransmitter, glutamate. The psychotropic cannabinoid receptor agonist Δ9-tetrahydrocannabinol (THC) and cannabidiol, (a non-psychoactive constituent of marijuana), both reduced NMDA, AMPA and kainate receptor mediated neurotoxicities. Neuroprotection was not affected by cannabinoid receptor antagonist, indicating a (cannabinoid) receptor-independent mechanism of action. Glutamate toxicity can be reduced by antioxidants. Using cyclic voltametry and a fenton reaction based system, it was demonstrated that Cannabidiol, THC and other cannabinoids are potent antioxidants. As evidence that cannabinoids can act as an antioxidants in neuronal cultures, cannabidiol was demonstrated to reduce hydroperoxide toxicity in neurons. In a head to head trial of the abilities of various antioxidants to prevent glutamate toxicity, cannabidiol was superior to both a-tocopherol and ascorbate in protective capacity. Recent preliminary studies in a rat model of focal cerebral ischemia suggest that cannabidiol may be at least as effective in vivo as seen in these in vitro studies.