Identification of Rickettsia spp. and Bartonella spp. in Fleas from the Thai-Myanmar Border

Authors

  • P. PAROLA,

    1. Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand
    2. Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Marseilles, France
    3. Laboratory of Public Health Entomology, Harvard School of Public Health, Boston, Massachusetts, USA
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  • O. Y. SANOGO,

    1. Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Marseilles, France
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  • K. LERDTHUSNEE,

    1. Department of Entomology, AFRIMS, Bangkok, Thailand
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  • Z. ZEAITER,

    1. Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Marseilles, France
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  • G. CHAUVANCY,

    1. Institut de Recherche pour le Développement UR34, Mahidol University at Salaya, Nakhon Pathom, Thailand
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  • J. P. GONZALEZ,

    1. Institut de Recherche pour le Développement UR34, Mahidol University at Salaya, Nakhon Pathom, Thailand
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  • R. S. MILLER,

    1. Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand
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  • S. R. TELFORD III,

    1. Laboratory of Public Health Entomology, Harvard School of Public Health, Boston, Massachusetts, USA
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  • C. WONGSRICHANALAI,

    1. Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand
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  • D. RAOULT

    Corresponding author
    1. Laboratory of Public Health Entomology, Harvard School of Public Health, Boston, Massachusetts, USA
      Address for correspondence: Didier Raoult, Unité des Rickettsies, CNRS UMR 6020, IFR 48, WHO Collaborative Center for Rickettsial Reference and Research, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385 Marseilles Cedex 5, France. Voice: 33-4-91-32-43-75; fax: 33-4-91-83-03-90. Didier.Raoult@medecine.univ-mrs.fr.
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Address for correspondence: Didier Raoult, Unité des Rickettsies, CNRS UMR 6020, IFR 48, WHO Collaborative Center for Rickettsial Reference and Research, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385 Marseilles Cedex 5, France. Voice: 33-4-91-32-43-75; fax: 33-4-91-83-03-90. Didier.Raoult@medecine.univ-mrs.fr.

Abstract

Abstract: During a survey for possible rickettsial vectors in villages of the central part of the Thai-Myanmar border from September 2001 to February 2002, four species of fleas were collected from common peridomestic animals. All fleas were tested by PCR to detect DNA of bacteria of the genera Rickettsia (gltA and ompB genes) and Bartonella (ITS and ftsZ genes). Sequencing of PCR-amplified products was done using gltA fragments for Rickettsia and ftsZ fragments for Bartonella. Two genotypes related to Rickettsia felis were identified in three Ctenocephalides canis and one C. felis specimen. Further, the following Bartonella spp. were detected: Bartonella henselae in two C. felis specimens; Bartonella clarridgeiae in three C. felis specimens; and a new Bartonella genotype in one Nosopsylla fasciatus specimen. Rickettsia and Bartonella may be frequently detected in fleas infesting peridomestic animals from the western border of Thailand.

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