INSL3 Plays a Role in the Balance between Bone Formation and Resorption

Authors

  • Anastasia Pepe,

    1. Section of Clinical Pathology & Centre for Male Gamete Cryopreservation, Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Padova, Italy
    Search for more papers by this author
  • Alberto Ferlin,

    1. Section of Clinical Pathology & Centre for Male Gamete Cryopreservation, Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Padova, Italy
    Search for more papers by this author
  • Lisa Gianesello,

    1. Section of Clinical Pathology & Centre for Male Gamete Cryopreservation, Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Padova, Italy
    Search for more papers by this author
  • Arianna Facciolli,

    1. Section of Clinical Pathology & Centre for Male Gamete Cryopreservation, Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Padova, Italy
    Search for more papers by this author
  • Alexander I. Agoulnik,

    1. Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas, USA
    Search for more papers by this author
  • Carlo Foresta

    1. Section of Clinical Pathology & Centre for Male Gamete Cryopreservation, Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Padova, Italy
    Search for more papers by this author

Address for correspondence: Prof. Carlo Foresta, University of Padova, Department of Histology, Microbiology and Medical Biotechnologies, Section of Clinical Pathology & Centre for Male Gamete Cryopreservation, Via Gabelli 63, 35121 Padova, Italy. Voice: +39 049 8218517; fax: +39 049 8218520. carlo.foresta@unipd.it

Abstract

We have recently demonstrated that the disruption of the INSL3 hormonal system, as observed in humans with mutations in the RXFP2 gene and in Rxfp2-deficient mice, might affect the equilibrium of the osteoblast–osteoclast system, resulting in an imbalance between bone formation and bone resorption that may result in reduced bone mass. In the present study we have better characterized the in vitro effects of INSL3 on human osteoblasts. Stimulation of human primary osteoblasts with INSL3 at serial concentrations (1 pM, 1 nM, and 1 μM) induced a dose-dependent increase in osteoblast proliferation and expression of specific osteoblast genes.

Ancillary