New Developments in Serodiagnosis of Childhood Celiac Disease

Assay of Antibodies against Deamidated Gliadin

  1. Christian Prause1,
  2. Thomas Richter2,
  3. Sibylle Koletzko3,
  4. H. Holm Uhlig4,
  5. Almuthe C. Hauer5,
  6. Martin Stern6,
  7. Klaus-Peter Zimmer7,
  8. Martin W. Laass8,
  9. Christian Probst9,
  10. Wolfgang Schlumberger9,
  11. Thomas Mothes1

Article first published online: 1 SEP 2009

DOI: 10.1111/j.1749-6632.2009.04638.x

Issue

Annals of the New York Academy of Sciences

Annals of the New York Academy of Sciences

Additional Information

How to Cite

Prause, C., Richter, T., Koletzko, S., Uhlig, H. H., Hauer, A. C., Stern, M., Zimmer, K.-P., Laass, M. W., Probst, C., Schlumberger, W. and Mothes, T. (2009), New Developments in Serodiagnosis of Childhood Celiac Disease. Annals of the New York Academy of Sciences, 1173: 28–35. doi: 10.1111/j.1749-6632.2009.04638.x

Author Information

  1. 1

    Institute of Laboratory Medicine, University Hospital Leipzig, Leipzig, Germany

  2. 2

    Department of Pediatrics of Municipal Hospital Sankt Georg Leipzig, Germany

  3. 3

    Dr. v. Haunersches Kinderspital, University Munich, Germany

  4. 4

    University Children's Hospital, Leipzig, Germany

  5. 5

    University Children's Hospital, Graz, Austria

  6. 6

    University Children's Hospital, Tuebingen, Germany

  7. 7

    University Children's Hospital, Giessen, Germany

  8. 8

    University Children's Hospital, Dresden, Germany

  9. 9

    EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany

*Address for correspondence: Professor Dr. Thomas Mothes, Institute for Laboratory Medicine, University Hospital and Medical Faculty of the University, Liebigstrasse 27, D-04103 Leipzig, Germany. mothes@medizin.uni-leipzig.de

Publication History

  1. Issue published online: 1 SEP 2009
  2. Article first published online: 1 SEP 2009

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Keywords:

  • accuracy;
  • celiac disease;
  • deamidated gliadin;
  • diagnosis;
  • tissue transglutaminase

Antibodies to deamidated gliadin present a new tool in the diagnosis of celiac disease (CD). In children, the ELISA for the determination of IgG antibodies to (deamidated) gliadin-analogous fusion peptides (GAF3X) has a superior performance compared to the ELISA for the determination of antibodies against native gliadin and is comparable to assays for IgA antibodies against tissue transglutaminase (IgA-anti-tTG). The combined investigation of IgG antibodies to GAF3X (IgG-anti-GAF3X) and IgA-anti-tTG significantly increases the fraction of children definitely identified as either CD or non-CD patients. The new IgG-anti-GAF3X ELISA was also able to detect CD in three cases of IgA deficiency and in two cases of latent CD and was also useful in the diagnosis of children younger than 2 years of age.

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