Omics approaches in cystic fibrosis research: a focus on oxylipin profiling in airway secretions
Article first published online: 3 JUL 2012
© 2012 New York Academy of Sciences.
Annals of the New York Academy of Sciences
Volume 1259, Environmental Stressors in Biology and Medicine pages 1–9, July 2012
How to Cite
Eiserich, J. P., Yang, J., Morrissey, B. M., Hammock, B. D. and Cross, C. E. (2012), Omics approaches in cystic fibrosis research: a focus on oxylipin profiling in airway secretions. Annals of the New York Academy of Sciences, 1259: 1–9. doi: 10.1111/j.1749-6632.2012.06580.x
- Issue published online: 3 JUL 2012
- Article first published online: 3 JUL 2012
- cystic fibrosis;
- respiratory tract secretions
Cystic fibrosis (CF) is associated with abnormal lipid metabolism, intense respiratory tract (RT) infection, and inflammation, eventually resulting in lung tissue destruction and respiratory failure. The CF RT inflammatory milieu, as reflected by airway secretions, includes a complex array of inflammatory mediators, bacterial products, and host secretions. It is dominated by neutrophils and their proteolytic and oxidative products and includes a wide spectrum of bioactive lipids produced by both host and presumably microbial metabolic pathways. The fairly recent advent of “omics” technologies has greatly increased capabilities of further interrogating this easily obtainable RT compartment that represents the apical culture media of the underlying RT epithelial cells. This paper discusses issues related to the study of CF omics with a focus on the profiling of CF RT oxylipins. Challenges in their identification/quantitation in RT fluids, their pathways of origin, and their potential utility for understanding CF RT inflammatory and oxidative processes are highlighted. Finally, the utility of oxylipin metabolic profiling in directing optimal therapeutic approaches and determining the efficacy of various interventions is discussed.