Effects of quercetin studied in colonic HT-29/B6 cells and rat intestine in vitro
Version of Record online: 25 JUN 2012
© 2012 New York Academy of Sciences.
Annals of the New York Academy of Sciences
Volume 1258, Barriers and Channels Formed by Tight Junction Proteins II pages 100–107, July 2012
How to Cite
Amasheh, M., Luettig, J., Amasheh, S., Zeitz, M., Fromm, M. and Schulzke, J.-D. (2012), Effects of quercetin studied in colonic HT-29/B6 cells and rat intestine in vitro. Annals of the New York Academy of Sciences, 1258: 100–107. doi: 10.1111/j.1749-6632.2012.06609.x
- Issue online: 25 JUN 2012
- Version of Record online: 25 JUN 2012
- barrier function;
- tight junction;
The aim of this study was to analyze the influence of quercetin on intestinal barrier function using the human colonic epithelial cell line HT-29/B6 and rat small and large intestine in vitro. Rat native ileum and late distal colon were incubated in Ussing chambers, and the total resistance (RT) was measured, and expression of tight junction proteins was characterized in immunoblots. By simulating inflammatory conditions with TNF-α, we examined the barrier-preventive effects of quercetin. Incubation with TNF-α led to a decrease of RT in HT-29/B6 cell monolayers, which could be partially inhibited by quercetin. In accordance with cell culture experiments, quercetin increased mucosal resistance of rat ileum and late distal colon. Thus, barrier disturbance in late distal colon specimens induced by TNF-α and IFN-γ could be partially prevented by coincubation with quercetin. These findings demonstrate that quercetin enhances barrier function in rat small and large intestine and possesses protective effects on cytokine-induced barrier damage.