Get access

Effects of KK-42 on Expression of 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase in Penaeus schmitti

Authors


Corresponding author.

Abstract

Methyl farnesoate (MF) is an important hormone controlling crustacean growth. Our previous study has demonstrated that the treatment of juvenile hormone antagonist KK-42 can accelerate growth of Penaeus schmitti. The expression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR), a key enzyme of MF precursor production, was assayed using real-time PCR, and hemolymph MF titer was determined by high-performance liquid chromatography. Shrimp, 3.5–5.0 cm long, were soaked for 1 min in KK-42 solution at a concentration of 1.95 × 10−4 mol/L or in the solution without KK-42 and then cultured in normal way. Results showed that the highest level of hmgr transcript occurred in mandibular organ (MO). Administration of KK-42 resulted in HMGR expression to be notably depressed in the MO, even reduced by 80.2% (P < 0.01) and 90.7% (P < 0.01) on Day 1 and Day 7, respectively. The MF level showed a decline trend in KK-42 treatment compared to that of corresponding control group. Our present study demonstrates that treatment of KK-42 significantly inhibits HMGR expression in different tissues especially in the MO, which is likely one of KK-42 growth-promoting mechanisms on P. schmitti.

Ancillary