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This study was conducted to determine the effects of the use of farmed salmon oil (FSO) as a substitute for anchovy oil (AO) on growth and nutrient utilization performance, and proximate and fatty acid compositions of turbot, Psetta maxima. Two fish oil sources, AO and FSO, were incorporated into the diet at a level of 6.77% and fed to turbot with an initial average weight of 266.25 ± 0.53 g in brackish water for 9 wks. Each diet was tested in four replicates and fed to apparent satiation twice a day. At the end of the experiment, data of growth and nutrient utilization performances (feed conversion ratio, nitrogen, lipid and energy intakes, gains, and retentions), and organo-somatic indices were similar between the dietary oil sources. Likewise, there were no significant differences in final whole body proximate compositions between two treatments. However, AO and FSO diets significantly affected whole body fatty acids and fatty acids classes. Briefly, FSO fish was characterized with higher n-6 poly unsaturated fatty acids (PUFA) mainly 18:2n-6 whereas AO fish with higher n-3 PUFA and highly unsaturated fatty acids mainly 22:6n-3. The whole body fatty acid profile reflected that of the diets. It is concluded that incorporation of FSO instead of AO in turbot diets supports similar growth and nutrient utilization performances but change whole body fatty acid profile.