Quantification of dust-borne transmission of highly pathogenic avian influenza virus between chickens
Version of Record online: 6 APR 2012
© 2012 Blackwell Publishing Ltd
Influenza and Other Respiratory Viruses
Volume 7, Issue 2, pages 132–138, March 2013
How to Cite
Spekreijse, D., Bouma, A., Koch, G. and Stegeman, A. (2013), Quantification of dust-borne transmission of highly pathogenic avian influenza virus between chickens. Influenza and Other Respiratory Viruses, 7: 132–138. doi: 10.1111/j.1750-2659.2012.00362.x
- Issue online: 11 FEB 2013
- Version of Record online: 6 APR 2012
- Accepted 24 February 2012. Published online 6 April 2012.
- Airborne transmission;
- highly pathogenic avian influenza virus
Please cite this paper as: Spekreijse et al. (2013) Quantification of dust-borne transmission of highly pathogenic avian influenza virus between chickens. Influenza and Other Respiratory Viruses 7(2) 132–138.
Background Understanding the transmission of highly pathogenic avian influenza virus (HPAIv) between poultry flocks is essential to prevent and control epidemics. Dust, produced in infected chicken flocks, has been hypothesized to be an important mechanical vector for between-flock transmission of HPAIv.
Objectives The aim of our study was to quantify the amount of virus shed by infected birds and its relation to deposition of virus in the environment and the rate of dust-borne transmission between groups of chickens.
Methods Four replicate experiments were performed, each replicate with two groups of 14 chickens housed in two separate rooms. In one group, chickens were inoculated with HPAIv. Ventilation forced the air from that room to the second (recipient) group through a tube. Deceased birds in the inoculated group were replaced with new susceptible birds up to day 10 p.i. Dust samples were collected daily. Trachea and cloaca swabs were collected daily to determine virus shedding and virus spread to the recipient group.
Results The amount of virus detected in dust samples in the recipient room was, on average, 103·7 EID50/m3. Virus transmission from the inoculated to the recipient group occurred in two experiments. The transmission rate parameter for dust-borne transmission was estimated at 0·08 new infections/infectious chicken/day.
Conclusions The results of this study are a first step to elucidate the importance of dust-borne transmission of HPAIv between flocks and help interpret environmental samples.