• 1
    Alexander DJ, Capua I, Koch G. Highly pathogenic avian influenza outbreaks in Europe, Asia and Africa since 1959, excluding the asian H5N1 virus outbreaks; in Swayne DE, (ed.): Avian Influenza Ames. Iowa: Blackwell Publishing, 2008; 217.
  • 2
    World Health Organization. H5N1 avian influenza: timeline of major events, 2011. Available at (Accessed 8 June 2011).
  • 3
    Lupiani B, Reddy SM. The history of avian influenza. Comp Immunol Microbiol Infect Dis2009; 32:311323.
  • 4
    Fouchier RAM, Osterhaus ADME, Brown IH. Animal influenza virus surveillance. Vaccine2003; 21:17541757.
  • 5
    Spickler AR, Trampel DW, Roth JA. The onset of virus shedding and clinical signs in chickens infected with high-pathogenicity and low-pathogenicity avian influenza viruses. Avian Pathol2008; 37:555577.
  • 6
    European Commission. Commission Decision 2010/367/EC on the implementation by member states of surveillance programmes for avian influenza in poultry and wild birds. Off J Eur Union2010; 166:2232.
  • 7
    European Commission. Commission Decision 2006/437/EC of 4 August 2006 approving a Diagnostic Manual for avian influenza as provided for Council Directive 2005/94/EC. Off J Eur Union2006; 237:1.
  • 8
    World Organization for Animal Health. Avian influenza; in Manual of Diagnostic Tests and Vaccines for Terrestrial Animals2009. Available at (Accessed 8 June 2011).
  • 9
    Yamamoto T, Tsutsui T, Nishiguchi A et al. Preliminary evaluation of diagnostic tests for avian influenza using the Markov Chain Monte Carlo (MCMC) Method in an emergency surveillance. J Vet Med Sci2007; 69:673675.
  • 10
    Greiner M, Gardner I. Epidemiologic issues in the validation of veterinary diagnostic tests. Prev Vet Med2000; 45:322.
  • 11
    Hui SL, Walter SD. Estimating the error rates of diagnostic tests. Biometrics1980; 36:167171.
  • 12
    Watcharatanyatip K, Boonmoh S, Chaichoun K, Songserm T, Woratanti M, Dharakul T. Multispecies detection of antibodies to influenza A viruses by a double-antigen sandwich ELISA. J Virol Methods2010; 163:238243.
  • 13
    Sala G, Cordioli P, Moreno-Martin A et al. ELISA test for the detection of influenza H7 antibodies in avian sera. Avian Dis2003; 3(Suppl.):10571059.
  • 14
    Dlugolenski D, Hauck R, Hogan RJ, Michel F, Mundt E. Production of H5-specific monoclonal antibodies and the development of a competitive enzyme-linked immunosorbent assay for detection of H5 antibodies in multiple species. Avian Dis2010; 1(Suppl):644649.
  • 15
    Cattoli G, Terregino C, Brasola V, Rodriguez JF, Capua I. Development and preliminary validation of an ad hoc N1-N3 discriminatory test for the control of avian influenza in Italy. Avian Dis2003; 3(Suppl.):10601062.
  • 16
    Chen Y, Chen C, Wang C. H5 antibody detection by blocking enzyme-linked immunosorbent assay using a monoclonal antibody. Avian Dis2008; 52:124129.
  • 17
    Chen Y, Juang R, He J, Chu W, Wang C. Detection of H6 influenza antibody by blocking enzyme-linked immunosorbent assay. Vet Microbiol2010; 142:205210.
  • 18
    Jin M, Wang G, Zhang R et al. Development of enzyme-linked immunosorbent assay with nucleoprotein as antigen for detection of antibodies to avian influenza virus. Avian Dis2004; 48:870878.
  • 19
    Branscum AJ, Gardner IA, Johnson WO. Estimation of diagnostic-test sensitivity and specificity through Bayesian modeling. Prev Vet Med2005; 68:145163.
  • 20
    Spiegelhalter D, Thomas A, Best N, Lunn D. WinBUGS User Manual – upgraded version 1.4.3., 2007. Available at (Accessed 1 July 2011).
  • 21
    Toft N, Innocent GT, Gettinby G, Reid SWJ. Assessing the convergence of Markov Chain Monte Carlo methods: an example from evaluation of diagnostic tests in absence of a gold standard. Prev Vet Med2007; 79:244256.
  • 22
    Van der Goot JA, Engel B, Van de Water SGP et al. Validation of diagnostic tests for detection of avian influenza in vaccinated chickens using Bayesian analysis. Vaccine2010; 28:17711777.
  • 23
    Upadhyay C, Ammayappan A, Vakharia VN. Detection of NP, N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast-expressed antigens. Virol J2009; 6:158.
  • 24
    Spiegelhalter DJ, Best NG, Carlin BP, Van Der Linde A. Bayesian measures of model complexity and fit. J R Statist Soc B2002; 64:583639.
  • 25
    Escorcia M, Carrillo-Sánchez K, March-Mifsut S, Chapa J, Lucio E, Nava G. Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses. BMC Vet Res2010; 6:57.
  • 26
    Marché S, Van den Berg T. Evaluation of different strategies for the use of ELISA tests as first screening tools for serologic surveillance of low pathogenic avian influenza in the Belgian poultry sector. Avian Dis2010; 54:627631.
  • 27
    Brown JD, Stallknecht DE, Berghaus RD et al. Evaluation of a commercial blocking enzyme-linked immunosorbent assay to detect avian influenza virus antibodies in multiple experimentally infected avian species. Clin Vaccine Immunol2009; 16:824829.
  • 28
    Cattoli G, Milani A, Bettini F et al. Development and validation of an anti-N3 indirect immunofluorescent antibody test to be used as a companion diagnostic test in the framework of a ‘DIVA’ vaccination strategy for avian influenza infections in poultry. Avian Pathol2006; 35:154159.
  • 29
    Song DS, Lee YJ, Jeong OM et al. Evaluation of a competitive ELISA for antibody detection against avian influenza virus. J Vet Sci2009; 10:323329.
  • 30
    Toft N, Jørgensen E, Gettinby G, Højsgaard S. Diagnosing diagnostic tests: evaluating the assumptions underlying the estimation of sensitivity and specificity in the absence of a gold standard. Prev Vet Med, 2005; 68:1933.