Demonstration of Amyloid Deposits and Neurofibrillary Changes in Whole Brain Sections
Article first published online: 28 JAN 2008
Volume 1, Issue 3, pages 213–216, April 1991
How to Cite
Braak, H. and Braak, E. (1991), Demonstration of Amyloid Deposits and Neurofibrillary Changes in Whole Brain Sections. Brain Pathology, 1: 213–216. doi: 10.1111/j.1750-3639.1991.tb00661.x
- Issue published online: 28 JAN 2008
- Article first published online: 28 JAN 2008
Selective and sensitive silver staining of extracellular amyloid deposits and intraneuronal neurofibrillary changes can be applied to 50–150 μ.m thick polyethylene glycol sections and/or 5–15 μ.m thick paraffin sections. The silver techniques take advantage of physical development of the nucleation sites thus permitting tight control of the entire procedure. Both techniques can be applied to routinely fixed autopsy material. They do not require particular skills and considerably facilitate processing of large numbers of sections through entire hemispheres of the human brain.