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NG2+/Olig2+ Cells are the Major Cycle-Related Cell Population of the Adult Human Normal Brain

Authors

  • Sameh Geha,

    1. Department of Neuropathology, Sainte-Anne Hospital, Paris, France.
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  • Johan Pallud,

    1. Department of Neurosurgery, Sainte-Anne Hospital, Paris, France.
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  • Marie-Pierre Junier,

    1. Department of Neuropathology, Sainte-Anne Hospital, Paris, France.
    2. UMR Inserm U894, Psychiatry and Neurosciences Center, University Descartes Paris 5, France.
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  • Bertrand Devaux,

    1. Department of Neurosurgery, Sainte-Anne Hospital, Paris, France.
    2. UMR Inserm U894, Psychiatry and Neurosciences Center, University Descartes Paris 5, France.
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  • Nadine Leonard,

    1. Department of Neuropathology, Sainte-Anne Hospital, Paris, France.
    2. UMR Inserm U894, Psychiatry and Neurosciences Center, University Descartes Paris 5, France.
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  • Francine Chassoux,

    1. Department of Neurosurgery, Sainte-Anne Hospital, Paris, France.
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  • Hervé Chneiweiss,

    1. UMR Inserm U894, Psychiatry and Neurosciences Center, University Descartes Paris 5, France.
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  • Catherine Daumas-Duport,

    1. Department of Neuropathology, Sainte-Anne Hospital, Paris, France.
    2. UMR Inserm U894, Psychiatry and Neurosciences Center, University Descartes Paris 5, France.
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  • Pascale Varlet

    Corresponding author
    1. Department of Neuropathology, Sainte-Anne Hospital, Paris, France.
    2. UMR Inserm U894, Psychiatry and Neurosciences Center, University Descartes Paris 5, France.
      Pascale Varlet, MD, PhD, Department of Neuropathology, Sainte-Anne Hospital, 1, Rue Cabanis, 75674 Paris, France (E-mail: p.varlet@ch-sainte-anne.fr)
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Pascale Varlet, MD, PhD, Department of Neuropathology, Sainte-Anne Hospital, 1, Rue Cabanis, 75674 Paris, France (E-mail: p.varlet@ch-sainte-anne.fr)

Abstract

A persistent cycling cell population in the normal adult human brain is well established. Neural stem cells or neural progenitors have been identified in the subventricular zone and the dentate gyrus subgranular layer (SGL), two areas of persistent neurogenesis. Cycling cells in other human normal brain areas, however, remains to be established. Here, we determined the distribution and identity of these cells in the cortex, the white matter and the hippocampal formation of adult patients with and without chronic temporal lobe epilepsy using immunohistochemistry for the cell cycle markers Ki-67 (Mib-1) and minichromosome maintenance protein 2. Rare proliferative neuronal precursors expressing the neuronal antigen neuronal nuclei were restricted to the SGL. In contrast, the oligodendrocyte progenitor cell markers Olig2 and the surface antigen NG2 were expressed by the vast majority of cycling cells scattered throughout the cortex and white matter of both control and epileptic patients. Most of these cycling cells were in early G1 phase, and were significantly more numerous in epileptic than in non-epileptic patients. These results provide evidence for a persistent gliogenesis in the human cortex and white matter that is enhanced in an epileptic environment.

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