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Figure S1. Representative proteome pattern of A172 cells transfected with miR-184 precursors revealed by 2D-DIGE. Image analysis and subsequent MS analyses led to the identification of 24 significantly regulated proteins.

Figure S2. Representative proteome pattern of T98G cells transfected with miR-184 precursors revealed by 2D-DIGE. Image analysis and subsequent MS analyses led to the identification of 54 significantly regulated proteins.

Figure S3. Representative proteome pattern of A172 cells transfected with miR-17 inhibitors revealed by 2D-DIGE. Image analysis and subsequent MS analyses led to the identification of 23 significantly regulated proteins.

Figure S4. Representative proteome pattern of T98G cells transfected with miR-17 inhibitors revealed by 2D-DIGE. Image analysis and subsequent MS analyses led to the identification of 48 significantly regulated proteins.

Figure S5. Expression of Akt2 protein in diffuse astrocytomas as compared to secondary glioblastomas. Western blots containing protein extracts from five diffuse astrocytomas (AII) and five secondary glioblastomas (sGBIV) were probed with antibodies against Akt2 and β-actin as a loading control. Note increased Akt2 protein levels in secondary glioblastomas as compared to the low-grade diffuse astrocytomas.

Table S1. List of investigated human microRNAs (miRNAs) that were detectable with the Applied Biosystems miRNA Early Access Kit.

Table S2. Primer sequences used for duplex polymerase chain reaction analysis of microRNA (miRNA) loci and expression analyses of putative miRNA targets.

Table S3. Potential target genes of miR-184 identified by expression profiling using Affymetrix Human Genome U133 plus 2.0 arrays. The mRNA expression profiles of glioma cells with miR-184 overexpression (pre-184) and glioma cells transfected with negative controls (pre-NC) were compared. The list contains targets of miR-184 as predicted by the mirBase target database with signal log ratios (pre-184/pre-NC) < −1 and change P values <0.05 in both investigated glioma cell lines (A172 and T98G).

Table S4. Differential protein expression caused by miR-184 overexpression. A172 and T98G cells were transfected with miR-184 precursors (pre-184) or negative controls (pre-NC). 2D-DIGE analyses were performed to identify differentially expressed proteins. Note that nucleophosmin 1 is a predicted target of miR-184.

Table S5. Differential protein expression caused by inhibition of miR-17. A172 and T98G cells were transfected with miR-17 inhibitors (anti-17) or negative controls (anti-NC). 2D-DIGE analyses were performed to identify differentially expressed proteins. Note that Pold2 is a predicted target of miR-17.

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