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Use of Murine Bioassay to Resolve Ovine Transmissible Spongiform Encephalopathy Cases Showing a Bovine Spongiform Encephalopathy Molecular Profile
Article first published online: 13 OCT 2011
© 2011 The Authors. Brain Pathology © 2011 International Society of Neuropathology
Volume 22, Issue 3, pages 265–279, May 2012
How to Cite
Beck, K. E., Sallis, R. E., Lockey, R., Vickery, C. M., Béringue, V., Laude, H., Holder, T. M., Thorne, L., Terry, L. A., Tout, A. C., Jayasena, D., Griffiths, P. C., Cawthraw, S., Ellis, R., Balkema-Buschmann, A., Groschup, M. H., Simmons, M. M. and Spiropoulos, J. (2012), Use of Murine Bioassay to Resolve Ovine Transmissible Spongiform Encephalopathy Cases Showing a Bovine Spongiform Encephalopathy Molecular Profile. Brain Pathology, 22: 265–279. doi: 10.1111/j.1750-3639.2011.00526.x
- Issue published online: 12 APR 2012
- Article first published online: 13 OCT 2011
- Accepted manuscript online: 15 SEP 2011 10:30AM EST
- Received 27 May 2011; accepted 9 August 2011.
- mouse bioassay;
- transmissible spongiform encephalopathy
Two cases of unusual transmissible spongiform encephalopathy (TSE) were diagnosed on the same farm in ARQ/ARQ PrP sheep showing attributes of both bovine spongiform encephalopathy (BSE) and scrapie. These cases, UK-1 and UK-2, were investigated further by transmissions to wild-type and ovine transgenic mice. Lesion profiles (LP) on primary isolation and subpassage, incubation period (IP) of disease, PrPSc immunohistochemical (IHC) deposition pattern and Western blot profiles were used to characterize the prions causing disease in these sheep. Results showed that both cases were compatible with scrapie. The presence of BSE was contraindicated by the following: LP on primary isolation in RIII and/or MR (modified RIII) mice; IP and LP after serial passage in wild-type mice; PrPSc deposition pattern in wild-type mice; and IP and Western blot data in transgenic mice. Furthermore, immunohistochemistry (IHC) revealed that each case generated two distinct PrPSc deposition patterns in both wild-type and transgenic mice, suggesting that two scrapie strains coexisted in the ovine hosts. Critically, these data confirmed the original differential IHC categorization that these UK-1 and UK-2 cases were not compatible with BSE.