Axonopathy Is Associated with Complex Axonal Transport Defects in a Model of Multiple Sclerosis
Article first published online: 17 NOV 2011
© 2011 The Authors; Brain Pathology © 2011 International Society of Neuropathology
Volume 22, Issue 4, pages 454–471, July 2012
How to Cite
Kreutzer, M., Seehusen, F., Kreutzer, R., Pringproa, K., Kummerfeld, M., Claus, P., Deschl, U., Kalkul, A., Beineke, A., Baumgärtner, W. and Ulrich, R. (2012), Axonopathy Is Associated with Complex Axonal Transport Defects in a Model of Multiple Sclerosis. Brain Pathology, 22: 454–471. doi: 10.1111/j.1750-3639.2011.00541.x
- Issue published online: 14 JUN 2012
- Article first published online: 17 NOV 2011
- Accepted manuscript online: 11 OCT 2011 08:06AM EST
- Received 9 July 2011 Accepted 8 September 2011Published Online Article Accepted11 October 2011
Figure S1. Theiler's murine encephalomyelitis virus spread in the spinal cord. A. Distribution of TMEV-positive cells in the ventromedial spinal cord at 28 dpi. TMEV was found predominantly in microglia/macrophages (arrows). Scale bar = 200 μm. B. Inset of A which shows TMEV-positive microglia/macrophages cells (arrows) and a positive axon (arrowhead). Scale bar = 50 μm. Abbreviations: G = gray matter; W = white matter.
Figure S2.TMEV-induced demyelination. (A,B) Luxol fast blue cresyl-echt violet-stained ventromedial spinal cord of control (A) and TMEV-infected mice (B) at 4, 42 and 98 dpi. At 4 dpi, the spinal cord of control and infected mice showed a uniformly Luxol fast blue-stained white matter. At 42 dpi, lymphohistiocytic (arrow) and mild demyelination of the white matter (asterisks) were present. At 98 dpi, a minimal diffuse parenchymal inflammatory reaction and lymphohistiocytic meningitis (arrows) and an increased area of demyelination can be observed (asterisks). Scale bar = 100 μm. Abbreviations: G = gray matter; W = white matter.
Figure S3.Axonal loss in TME. (A,B) Results of Bielschowsky silver stain in the ventromedial spinal cord of control (A) and TMEV-infected mice (B) at 0, 28, 98 and 196 dpi. At 0 dpi, normal staining of axons in the spinal cord of the control and TMEV-infected mice was seen. From 28 until 196 dpi extensive pathology of the nerve fibers characterized by variation of axonal thickness, loss of axons (arrows) and presence of spheroids (arrowheads) was observed in TMEV-infected mice compared with controls. Scale bar = 100 μm. Abbreviation: W = white matter.
Table S1. Axonal transport associated probe sets manually curated from peer-reviewed scientific articles.
Table S2. Axonal self-destruction associated probe sets manually curated from peer-reviewed scientific articles.
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