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Figure S1. Proteinase K digestion of cerebellar tissue containing PrPSc prion plaques. A. High power magnification (400×) of a PrPSc plaque immunoreactive with 3F4 antibody utilizing Histelide without Proteinase K digestion. B. High power magnification (400×) of a PrPSc plaque immunoreactive with 3F4 antibody utilizing Histelide following Proteinase K digestion as described in Methods. Note background tissue has been digested from glass slide while the PrPSc plaque was partially resistant to digestion. Due to extensive tissue digestion an air bubble is present in the right side of the image.

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