Figure S1. Schematic overview of different MCAO models and resulting infarct demarcation on histological sections. (A) Left: in transient MCAO caused by the intraluminal filament, the origin of the MCAO is occluded. Right: transtemporal electrocoagulation of the MCAO is performed beyond the branching of the lenticulostriate arteries. Site of occlusion is marked in red. ACA = anterior cerebral artery; MCA = middle cerebral artery; PCA = posterior cerebral artery; BA = basilar artery; ICA = internal carotid artery. (B) Resulting infarcts depend on the site of occlusion and the duration of ischemia. Representative cresyl-violett stained cryosection of the analyzed stroke models, infarct areas are encircled. Comparison of infarct volume (C) and mortality (D) at 24 h and 5 days among the MCAO models.


Figure S2. Schematic overview of the fields of interest (FOIs) for comparison of activated microglia; 6x FOIs (each 0.16 mm2) surrounding the infarct area in the ischemic hemisphere and corresponding areas in the nonischemic hemisphere were defined on the immunohistochemistry sections for Iba1 of the analyzed stroke models to evaluate the activated microglia.

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