Get access

Effects of Short-Chain Nitrocompounds against Campylobacter jejuni and Campylobacter coli in vitro

Authors

  • S.M. Horrocks,

    1. Authors Horrocks, Jung, Harvey, Callaway, Anderson, Ramlachan, and Nisbet are with the U.S. Dept. of Agriculture, Agricultural Research Service, Food & Feed Safety Research Unit, College Station, TX 77845. Authors Horrocks and Carstens are with the Intercollegiate Faculty of Nutrition, Texas A&M Univ., College Station, TX 77845. Author Huwe is with the U.S. Dept. of Agriculture, Agricultural Research Service, Biosciences Research Unit, Fargo, ND 77845. Author Ricke is with the Dept. of Poultry Science, Texas A&M Univ., College Station, TX 77845. Author Ricke is the Director of the Food Safety Center, Food Science Dept., Univ. of Arkansas, Fayetteville, AR 72704, U.S.A. Direct inquires to Anderson (E-mail: anderson@ffsru.tamu.edu).
    Search for more papers by this author
  • Y.S. Jung,

    1. Authors Horrocks, Jung, Harvey, Callaway, Anderson, Ramlachan, and Nisbet are with the U.S. Dept. of Agriculture, Agricultural Research Service, Food & Feed Safety Research Unit, College Station, TX 77845. Authors Horrocks and Carstens are with the Intercollegiate Faculty of Nutrition, Texas A&M Univ., College Station, TX 77845. Author Huwe is with the U.S. Dept. of Agriculture, Agricultural Research Service, Biosciences Research Unit, Fargo, ND 77845. Author Ricke is with the Dept. of Poultry Science, Texas A&M Univ., College Station, TX 77845. Author Ricke is the Director of the Food Safety Center, Food Science Dept., Univ. of Arkansas, Fayetteville, AR 72704, U.S.A. Direct inquires to Anderson (E-mail: anderson@ffsru.tamu.edu).
    Search for more papers by this author
  • J.K. Huwe,

    1. Authors Horrocks, Jung, Harvey, Callaway, Anderson, Ramlachan, and Nisbet are with the U.S. Dept. of Agriculture, Agricultural Research Service, Food & Feed Safety Research Unit, College Station, TX 77845. Authors Horrocks and Carstens are with the Intercollegiate Faculty of Nutrition, Texas A&M Univ., College Station, TX 77845. Author Huwe is with the U.S. Dept. of Agriculture, Agricultural Research Service, Biosciences Research Unit, Fargo, ND 77845. Author Ricke is with the Dept. of Poultry Science, Texas A&M Univ., College Station, TX 77845. Author Ricke is the Director of the Food Safety Center, Food Science Dept., Univ. of Arkansas, Fayetteville, AR 72704, U.S.A. Direct inquires to Anderson (E-mail: anderson@ffsru.tamu.edu).
    Search for more papers by this author
  • R.B. Harvey,

    1. Authors Horrocks, Jung, Harvey, Callaway, Anderson, Ramlachan, and Nisbet are with the U.S. Dept. of Agriculture, Agricultural Research Service, Food & Feed Safety Research Unit, College Station, TX 77845. Authors Horrocks and Carstens are with the Intercollegiate Faculty of Nutrition, Texas A&M Univ., College Station, TX 77845. Author Huwe is with the U.S. Dept. of Agriculture, Agricultural Research Service, Biosciences Research Unit, Fargo, ND 77845. Author Ricke is with the Dept. of Poultry Science, Texas A&M Univ., College Station, TX 77845. Author Ricke is the Director of the Food Safety Center, Food Science Dept., Univ. of Arkansas, Fayetteville, AR 72704, U.S.A. Direct inquires to Anderson (E-mail: anderson@ffsru.tamu.edu).
    Search for more papers by this author
  • S.C. Ricke,

    1. Authors Horrocks, Jung, Harvey, Callaway, Anderson, Ramlachan, and Nisbet are with the U.S. Dept. of Agriculture, Agricultural Research Service, Food & Feed Safety Research Unit, College Station, TX 77845. Authors Horrocks and Carstens are with the Intercollegiate Faculty of Nutrition, Texas A&M Univ., College Station, TX 77845. Author Huwe is with the U.S. Dept. of Agriculture, Agricultural Research Service, Biosciences Research Unit, Fargo, ND 77845. Author Ricke is with the Dept. of Poultry Science, Texas A&M Univ., College Station, TX 77845. Author Ricke is the Director of the Food Safety Center, Food Science Dept., Univ. of Arkansas, Fayetteville, AR 72704, U.S.A. Direct inquires to Anderson (E-mail: anderson@ffsru.tamu.edu).
    Search for more papers by this author
  • G.E. Carstens,

    1. Authors Horrocks, Jung, Harvey, Callaway, Anderson, Ramlachan, and Nisbet are with the U.S. Dept. of Agriculture, Agricultural Research Service, Food & Feed Safety Research Unit, College Station, TX 77845. Authors Horrocks and Carstens are with the Intercollegiate Faculty of Nutrition, Texas A&M Univ., College Station, TX 77845. Author Huwe is with the U.S. Dept. of Agriculture, Agricultural Research Service, Biosciences Research Unit, Fargo, ND 77845. Author Ricke is with the Dept. of Poultry Science, Texas A&M Univ., College Station, TX 77845. Author Ricke is the Director of the Food Safety Center, Food Science Dept., Univ. of Arkansas, Fayetteville, AR 72704, U.S.A. Direct inquires to Anderson (E-mail: anderson@ffsru.tamu.edu).
    Search for more papers by this author
  • T.R. Callaway,

    1. Authors Horrocks, Jung, Harvey, Callaway, Anderson, Ramlachan, and Nisbet are with the U.S. Dept. of Agriculture, Agricultural Research Service, Food & Feed Safety Research Unit, College Station, TX 77845. Authors Horrocks and Carstens are with the Intercollegiate Faculty of Nutrition, Texas A&M Univ., College Station, TX 77845. Author Huwe is with the U.S. Dept. of Agriculture, Agricultural Research Service, Biosciences Research Unit, Fargo, ND 77845. Author Ricke is with the Dept. of Poultry Science, Texas A&M Univ., College Station, TX 77845. Author Ricke is the Director of the Food Safety Center, Food Science Dept., Univ. of Arkansas, Fayetteville, AR 72704, U.S.A. Direct inquires to Anderson (E-mail: anderson@ffsru.tamu.edu).
    Search for more papers by this author
  • R.C. Anderson,

    1. Authors Horrocks, Jung, Harvey, Callaway, Anderson, Ramlachan, and Nisbet are with the U.S. Dept. of Agriculture, Agricultural Research Service, Food & Feed Safety Research Unit, College Station, TX 77845. Authors Horrocks and Carstens are with the Intercollegiate Faculty of Nutrition, Texas A&M Univ., College Station, TX 77845. Author Huwe is with the U.S. Dept. of Agriculture, Agricultural Research Service, Biosciences Research Unit, Fargo, ND 77845. Author Ricke is with the Dept. of Poultry Science, Texas A&M Univ., College Station, TX 77845. Author Ricke is the Director of the Food Safety Center, Food Science Dept., Univ. of Arkansas, Fayetteville, AR 72704, U.S.A. Direct inquires to Anderson (E-mail: anderson@ffsru.tamu.edu).
    Search for more papers by this author
  • N. Ramlachan,

    1. Authors Horrocks, Jung, Harvey, Callaway, Anderson, Ramlachan, and Nisbet are with the U.S. Dept. of Agriculture, Agricultural Research Service, Food & Feed Safety Research Unit, College Station, TX 77845. Authors Horrocks and Carstens are with the Intercollegiate Faculty of Nutrition, Texas A&M Univ., College Station, TX 77845. Author Huwe is with the U.S. Dept. of Agriculture, Agricultural Research Service, Biosciences Research Unit, Fargo, ND 77845. Author Ricke is with the Dept. of Poultry Science, Texas A&M Univ., College Station, TX 77845. Author Ricke is the Director of the Food Safety Center, Food Science Dept., Univ. of Arkansas, Fayetteville, AR 72704, U.S.A. Direct inquires to Anderson (E-mail: anderson@ffsru.tamu.edu).
    Search for more papers by this author
  • D.J. Nisbet

    1. Authors Horrocks, Jung, Harvey, Callaway, Anderson, Ramlachan, and Nisbet are with the U.S. Dept. of Agriculture, Agricultural Research Service, Food & Feed Safety Research Unit, College Station, TX 77845. Authors Horrocks and Carstens are with the Intercollegiate Faculty of Nutrition, Texas A&M Univ., College Station, TX 77845. Author Huwe is with the U.S. Dept. of Agriculture, Agricultural Research Service, Biosciences Research Unit, Fargo, ND 77845. Author Ricke is with the Dept. of Poultry Science, Texas A&M Univ., College Station, TX 77845. Author Ricke is the Director of the Food Safety Center, Food Science Dept., Univ. of Arkansas, Fayetteville, AR 72704, U.S.A. Direct inquires to Anderson (E-mail: anderson@ffsru.tamu.edu).
    Search for more papers by this author

  • Mention of trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA and does not imply its approval to the exclusion of other products that may be suitable.

Abstract

ABSTRACT:  Effects of 2-nitro-1-propanol, 2-nitroethanol, nitroethane, and 2-nitro-methyl-propionate (0, 10, and 20 mM) on growth of Campylobacter jejuni were tested during culture in Bolton broth adjusted to pH 5.6, 7.0, or 8.2. The nitrocompounds were similarly tested against C. coli but at pH 8.2 only. Viable cell counts measured during incubation revealed main effects (P < 0.05) of all nitrocompounds on the survivability of C. jejuni. An effect of pH (P < 0.05) on the survivability of C. Jejuni during incubation with nitrocompounds was observed, with greater inhibition observed at pH 8.2 than at pH 5.6 or 7.0 for nitroethane, 2-nitro-l-propanol, and 2-nitroethanol, but not for 2-nitro-methyl-propionate, which showed greatest inhibition at pH 5.6. Except for 2-nitro-methyl-propionate, which was ineffective, all nitrocompounds elicited similar effects on C. coli. The effect of nitroethane and 2-nitro-l-propanol (10 mM) on naturally occurring Campylobacter was investigated during incubation of porcine fecal suspensions, where Campylobacter concentrations decreased more rapidly (P < 0.05) in suspensions with added 2-nitro-l-propanol than in unsupplemented or nitroethane-supplemented suspensions, thus reiterating the superior inhibitory effect of 2-nitro-l-propanol.

Get access to the full text of this article

Ancillary