Inactivation of Enterobacter sakazakii in Infant Milk Formula by Gamma Irradiation: Determination of D10-Value

Authors

  • T.M. Osaili,

    1. Authors Osaili, Shaker, Abu Al- Hasan, and Ayyash are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, P.O. Box (3030) Irbid, 22110, Jordan. Author Martin is with Dept. of Biological and Agricultural Engineering, Univ. of Arkansas, Fayetteville, AR 72701. Direct inquires to author Osaili (E-mail: tosaili@just.edu.jo).
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  • R.R. Shaker,

    1. Authors Osaili, Shaker, Abu Al- Hasan, and Ayyash are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, P.O. Box (3030) Irbid, 22110, Jordan. Author Martin is with Dept. of Biological and Agricultural Engineering, Univ. of Arkansas, Fayetteville, AR 72701. Direct inquires to author Osaili (E-mail: tosaili@just.edu.jo).
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  • A.S. Abu Al-Hasan,

    1. Authors Osaili, Shaker, Abu Al- Hasan, and Ayyash are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, P.O. Box (3030) Irbid, 22110, Jordan. Author Martin is with Dept. of Biological and Agricultural Engineering, Univ. of Arkansas, Fayetteville, AR 72701. Direct inquires to author Osaili (E-mail: tosaili@just.edu.jo).
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  • M.M. Ayyash,

    1. Authors Osaili, Shaker, Abu Al- Hasan, and Ayyash are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, P.O. Box (3030) Irbid, 22110, Jordan. Author Martin is with Dept. of Biological and Agricultural Engineering, Univ. of Arkansas, Fayetteville, AR 72701. Direct inquires to author Osaili (E-mail: tosaili@just.edu.jo).
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  • E.M. Martin

    1. Authors Osaili, Shaker, Abu Al- Hasan, and Ayyash are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, P.O. Box (3030) Irbid, 22110, Jordan. Author Martin is with Dept. of Biological and Agricultural Engineering, Univ. of Arkansas, Fayetteville, AR 72701. Direct inquires to author Osaili (E-mail: tosaili@just.edu.jo).
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Abstract

ABSTRACT: Enterobacter sakazakii is an emerging foodborne pathogen that has caused several cases of meningitis and necrotizing entercolitis in infants and has been associated with infant formulas. Five strains of E. sakazakii were inoculated individually into brain heart infusion broth and rehydrated or dehydrated infant milk formula and exposed to ionizing radiation. E. sakazakii strains in brain heart infusion broth and rehydrated infant milk formula (RIMF) were exposed to irradiation dose of up to 1 kGy while strains in dehydrated infant milk formula (DIMF) were exposed to irradiation dose of up to 9 kGy. The D10-values were determined by using a linear regression model. Average calculated D10-values ranged from 0.21 to 0.29 kGy, 0.24 to 0.37 kGy, and 1.06 to 1.71 kGy in brain heart infusion broth, RIMF, and DIMF, respectively. The results obtained from this study will be useful for powdered infant milk formula industries to reduce the risk associated with E. sakazakii.

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