Synergistic Antiyeast Activity of Garlic Oil and Allyl Alcohol Derived from Alliin in Garlic

Authors

  • I. Chung,

    1. Authors Chung, Kwon, and Kyung are with Dept. of Food Science, Sejong Univ., Kwangjin-ku, Seoul 143-747, Korea. Author Shim is with R&D Center, Nong Shim Food Co. LTD., Kunpo-shi, Kyunggi-do 435-723, Korea. Direct inquiries to author Kyung (E-mail: kyungkh@sejong.ac.kr).
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  • S.H. Kwon,

    1. Authors Chung, Kwon, and Kyung are with Dept. of Food Science, Sejong Univ., Kwangjin-ku, Seoul 143-747, Korea. Author Shim is with R&D Center, Nong Shim Food Co. LTD., Kunpo-shi, Kyunggi-do 435-723, Korea. Direct inquiries to author Kyung (E-mail: kyungkh@sejong.ac.kr).
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  • S.-T. Shim,

    1. Authors Chung, Kwon, and Kyung are with Dept. of Food Science, Sejong Univ., Kwangjin-ku, Seoul 143-747, Korea. Author Shim is with R&D Center, Nong Shim Food Co. LTD., Kunpo-shi, Kyunggi-do 435-723, Korea. Direct inquiries to author Kyung (E-mail: kyungkh@sejong.ac.kr).
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  • K.H. Kyung

    1. Authors Chung, Kwon, and Kyung are with Dept. of Food Science, Sejong Univ., Kwangjin-ku, Seoul 143-747, Korea. Author Shim is with R&D Center, Nong Shim Food Co. LTD., Kunpo-shi, Kyunggi-do 435-723, Korea. Direct inquiries to author Kyung (E-mail: kyungkh@sejong.ac.kr).
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Abstract

ABSTRACT:  Garlic oil (GO) and allyl alcohol (AA), both derived from alliin in garlic using different preparation methods, cause potent growth inhibition in yeasts. GO and AA inhibited Candida utilis ATCC42416 in different ways: GO has fungistatic activity while AA has fungicidal activity. GO and AA have almost identical antimicrobial potencies against the indicator yeast, with minimum inhibitory concentrations of about 25 ppm when tested individually. When GO and AA were tested in combination, the antimicrobial efficacy significantly increased. Combinations of AA and GO at 1 and 9 ppm, 2 and 7 ppm, 5 and 5 ppm, and 6 and 3 ppm, respectively, inhibited C. utilis completely. The sum of the fractional inhibitory concentrations (FICs) in the 2-component (GO and AA) combination was as low as 0.37 for C. utilis, indicating strong synergism. The sum of the FICs of diallyl disulfide and diallyl trisulfide in combination with AA was larger than that of the GO–AA combination, suggesting that the synergy was lower.

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