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Effect of Pectin Enhancement on Plasma Quercetin and Fecal Flora in Rutin-Supplemented Mice

Authors

  • M. Tamura,

    1. Authors Tamura, Nakagawa, and Tsushida are with Natl. Food Research Inst., Tsukuba 305-8642, Japan. Authors Hirayama and Itoh are with Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo, Tokyo 113-8657, Japan. Direct inquiries to author Tamura (E-mail: motoita@affrc.go.jp).
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  • H. Nakagawa,

    1. Authors Tamura, Nakagawa, and Tsushida are with Natl. Food Research Inst., Tsukuba 305-8642, Japan. Authors Hirayama and Itoh are with Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo, Tokyo 113-8657, Japan. Direct inquiries to author Tamura (E-mail: motoita@affrc.go.jp).
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  • T. Tsushida,

    1. Authors Tamura, Nakagawa, and Tsushida are with Natl. Food Research Inst., Tsukuba 305-8642, Japan. Authors Hirayama and Itoh are with Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo, Tokyo 113-8657, Japan. Direct inquiries to author Tamura (E-mail: motoita@affrc.go.jp).
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  • K. Hirayama,

    1. Authors Tamura, Nakagawa, and Tsushida are with Natl. Food Research Inst., Tsukuba 305-8642, Japan. Authors Hirayama and Itoh are with Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo, Tokyo 113-8657, Japan. Direct inquiries to author Tamura (E-mail: motoita@affrc.go.jp).
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  • K. Itoh

    1. Authors Tamura, Nakagawa, and Tsushida are with Natl. Food Research Inst., Tsukuba 305-8642, Japan. Authors Hirayama and Itoh are with Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo, Tokyo 113-8657, Japan. Direct inquiries to author Tamura (E-mail: motoita@affrc.go.jp).
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Abstract

ABSTRACT:  Few reports have considered the effects of dietary fiber on plasma quercetin and the intestinal flora. We investigated the effects of pectin on the plasma and fecal flora of mice fed a diet supplemented with the quercetin glycoside rutin. Male mice were randomly divided into 2 groups, which were fed a pectin–rutin (PR) or cellulose–rutin (CR) diet for 14 d. Plasma quercetin and isorhamnetin metabolites were measured by high-performance liquid chromatography. Feces were immediately processed with bacteriological procedures. The fecal flora was investigated. Plasma quercetin and isorhamnetin concentrations were significantly higher in the PR diet group, as was the plasma isorhamnetin/quercetin ratio. The composition of the intestinal flora differed between the 2 dietary groups. The total number of fecal bacteria was significantly larger in the PR group, in which most types of bacteria were more abundant, with the exceptions of bifidobacteria, fusiform-shaped bacteria, and staphylococci. The lower gut seemed to be the major absorption site for rutin. Pectin might thus enhance the bioavailability of quercetin from rutin by altering the metabolic activity of the intestinal flora and/or gut physiological function.

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