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Antiallergic Activities of Pigmented Rice Bran Extracts in Cell Assays

Authors

  • Sun Phil Choi,

    1. Authors Choi and Nam are with Dept. of Biological Science, Ajou Univ., Suwon 443-749, Korea. Author Kang is with Dept. of Food Science and Nutrition, Kyungpook Natl. Univ., Daegu 702-701, Korea. Author Koh is with Dept. of Agronomy, Seoul Natl. Univ., Seoul 151-742, Korea. Author Friedman is with the Western Regional Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, Albany, CA 94710, U.S.A. Direct inquiries to authors Nam (E-mail: shnam@ajou.ac.kr) and Friedman (E-mail:mfried@pw.usda.gov). [Correction added after online publication 10/26/07: Author Friedman's first name was corrected to Mendel and author Nam's e-mail address was corrected to shnam@ajou.ac.kr]
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  • Mi Young Kang,

    1. Authors Choi and Nam are with Dept. of Biological Science, Ajou Univ., Suwon 443-749, Korea. Author Kang is with Dept. of Food Science and Nutrition, Kyungpook Natl. Univ., Daegu 702-701, Korea. Author Koh is with Dept. of Agronomy, Seoul Natl. Univ., Seoul 151-742, Korea. Author Friedman is with the Western Regional Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, Albany, CA 94710, U.S.A. Direct inquiries to authors Nam (E-mail: shnam@ajou.ac.kr) and Friedman (E-mail:mfried@pw.usda.gov). [Correction added after online publication 10/26/07: Author Friedman's first name was corrected to Mendel and author Nam's e-mail address was corrected to shnam@ajou.ac.kr]
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  • Hee Jong Koh,

    1. Authors Choi and Nam are with Dept. of Biological Science, Ajou Univ., Suwon 443-749, Korea. Author Kang is with Dept. of Food Science and Nutrition, Kyungpook Natl. Univ., Daegu 702-701, Korea. Author Koh is with Dept. of Agronomy, Seoul Natl. Univ., Seoul 151-742, Korea. Author Friedman is with the Western Regional Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, Albany, CA 94710, U.S.A. Direct inquiries to authors Nam (E-mail: shnam@ajou.ac.kr) and Friedman (E-mail:mfried@pw.usda.gov). [Correction added after online publication 10/26/07: Author Friedman's first name was corrected to Mendel and author Nam's e-mail address was corrected to shnam@ajou.ac.kr]
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  • Seok Hyun Nam,

    1. Authors Choi and Nam are with Dept. of Biological Science, Ajou Univ., Suwon 443-749, Korea. Author Kang is with Dept. of Food Science and Nutrition, Kyungpook Natl. Univ., Daegu 702-701, Korea. Author Koh is with Dept. of Agronomy, Seoul Natl. Univ., Seoul 151-742, Korea. Author Friedman is with the Western Regional Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, Albany, CA 94710, U.S.A. Direct inquiries to authors Nam (E-mail: shnam@ajou.ac.kr) and Friedman (E-mail:mfried@pw.usda.gov). [Correction added after online publication 10/26/07: Author Friedman's first name was corrected to Mendel and author Nam's e-mail address was corrected to shnam@ajou.ac.kr]
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  • Mendel Friedman

    1. Authors Choi and Nam are with Dept. of Biological Science, Ajou Univ., Suwon 443-749, Korea. Author Kang is with Dept. of Food Science and Nutrition, Kyungpook Natl. Univ., Daegu 702-701, Korea. Author Koh is with Dept. of Agronomy, Seoul Natl. Univ., Seoul 151-742, Korea. Author Friedman is with the Western Regional Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, Albany, CA 94710, U.S.A. Direct inquiries to authors Nam (E-mail: shnam@ajou.ac.kr) and Friedman (E-mail:mfried@pw.usda.gov). [Correction added after online publication 10/26/07: Author Friedman's first name was corrected to Mendel and author Nam's e-mail address was corrected to shnam@ajou.ac.kr]
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Abstract

ABSTRACT:  Using a panel of chemical, biochemical, and cell assays, we determined inhibitory effects of extracts of the pigmented black rice brans on in vitro allergic reactions. Ethanol-water (70% v/v) extracts from 5 pigmented brans were found to be more effective than an extract from a nonpigmented rice cultivar in suppressing the release of histamine and β-hexosaminidase from basophilic RBL-2H3 cells stimulated with both Ionophore A23187 and immunoglobulin E (IgE)-antigen complexes. Suppression was also obtained with A23187-stimulated rat peritoneal mast cells. The extent of inhibition of these 2 markers of the immune response was accompanied by an influx of calcium ions. The inhibition of the immune process by the pigmented brans was confirmed by the observed modulation of the proinflammatory cytokine gene expressions and cytokine release, as indicated by the reduction in tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-4, and IL-6 mRNA expressions determined with the reverse transcription-polymerase chain reaction (RT-PCR). Reduction of TNF-α, IL-1β, and IL-6 protein release from both the cultured cell line and peritoneal cells was further confirmed by enzyme-linked immunoadsorbent assays. Rice bran from the LK1-3-6-12-1-1 cultivar was the most effective inhibitor in all assays. This particular rice variety merits further evaluation as part of a human diet to ascertain its potential to protect against allergic diseases such as hay fever and asthma.

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