Heat-Moisture Treatment of High-Amylose Corn Starch Increases Dietary Fiber Content and Lowers Plasma Cholesterol in Ovariectomized Rats

Authors

  • X. Liu,

    1. Authors Liu, Kishida, and Ebihara are with Dept. of Biological Resources, Faculty of Agriculture, Ehime Univ., Matsuyama 790-8566, Japan. Author Ogawa is with Faculty of Human and Cultural Studies, Tezukayamagakuin Univ., 4-2-2 Harumidai, Minami-ku, Sakai-city, Osaka 590-0113, Japan. Authors Ando and Nakakuki are with Research Inst., Nihon Shokuhin Kako Co., Ltd., 30 Tajima, Fuji, Shizuoka, 417-8530, Japan. Direct inquiries to author Ebihara (E-mail: ebihara@agr.ehime-u.ac.jp).
    Search for more papers by this author
  • H. Ogawa,

    1. Authors Liu, Kishida, and Ebihara are with Dept. of Biological Resources, Faculty of Agriculture, Ehime Univ., Matsuyama 790-8566, Japan. Author Ogawa is with Faculty of Human and Cultural Studies, Tezukayamagakuin Univ., 4-2-2 Harumidai, Minami-ku, Sakai-city, Osaka 590-0113, Japan. Authors Ando and Nakakuki are with Research Inst., Nihon Shokuhin Kako Co., Ltd., 30 Tajima, Fuji, Shizuoka, 417-8530, Japan. Direct inquiries to author Ebihara (E-mail: ebihara@agr.ehime-u.ac.jp).
    Search for more papers by this author
  • R. Ando,

    1. Authors Liu, Kishida, and Ebihara are with Dept. of Biological Resources, Faculty of Agriculture, Ehime Univ., Matsuyama 790-8566, Japan. Author Ogawa is with Faculty of Human and Cultural Studies, Tezukayamagakuin Univ., 4-2-2 Harumidai, Minami-ku, Sakai-city, Osaka 590-0113, Japan. Authors Ando and Nakakuki are with Research Inst., Nihon Shokuhin Kako Co., Ltd., 30 Tajima, Fuji, Shizuoka, 417-8530, Japan. Direct inquiries to author Ebihara (E-mail: ebihara@agr.ehime-u.ac.jp).
    Search for more papers by this author
  • T. Nakakuki,

    1. Authors Liu, Kishida, and Ebihara are with Dept. of Biological Resources, Faculty of Agriculture, Ehime Univ., Matsuyama 790-8566, Japan. Author Ogawa is with Faculty of Human and Cultural Studies, Tezukayamagakuin Univ., 4-2-2 Harumidai, Minami-ku, Sakai-city, Osaka 590-0113, Japan. Authors Ando and Nakakuki are with Research Inst., Nihon Shokuhin Kako Co., Ltd., 30 Tajima, Fuji, Shizuoka, 417-8530, Japan. Direct inquiries to author Ebihara (E-mail: ebihara@agr.ehime-u.ac.jp).
    Search for more papers by this author
  • T. Kishida,

    1. Authors Liu, Kishida, and Ebihara are with Dept. of Biological Resources, Faculty of Agriculture, Ehime Univ., Matsuyama 790-8566, Japan. Author Ogawa is with Faculty of Human and Cultural Studies, Tezukayamagakuin Univ., 4-2-2 Harumidai, Minami-ku, Sakai-city, Osaka 590-0113, Japan. Authors Ando and Nakakuki are with Research Inst., Nihon Shokuhin Kako Co., Ltd., 30 Tajima, Fuji, Shizuoka, 417-8530, Japan. Direct inquiries to author Ebihara (E-mail: ebihara@agr.ehime-u.ac.jp).
    Search for more papers by this author
  • K. Ebihara

    1. Authors Liu, Kishida, and Ebihara are with Dept. of Biological Resources, Faculty of Agriculture, Ehime Univ., Matsuyama 790-8566, Japan. Author Ogawa is with Faculty of Human and Cultural Studies, Tezukayamagakuin Univ., 4-2-2 Harumidai, Minami-ku, Sakai-city, Osaka 590-0113, Japan. Authors Ando and Nakakuki are with Research Inst., Nihon Shokuhin Kako Co., Ltd., 30 Tajima, Fuji, Shizuoka, 417-8530, Japan. Direct inquiries to author Ebihara (E-mail: ebihara@agr.ehime-u.ac.jp).
    Search for more papers by this author

Abstract

ABSTRACT:  The effect of dietary high-amylose corn starch (HACS) of varying dietary fiber (DF) content on plasma cholesterol was examined in ovariectomized (OVX) rats. Gelatinized normal corn starch (G-CS) was used as a reference. OVX rats were fed a fiber-free purified diet containing G-CS, HACS, gelatinized high-amylose corn starch (G-HACS), or heat-moisture treated HACS (HM-HACS) at 400 g starch/kg diet for 21 d. The DF content of G-CS, HACS, G-HACS, and HM-HACS measured by the AOAC method was 0.1, 19.3, 2.4, and 64.5 g/100 g, respectively. The dry weight of cecal contents, cecal wall weight, the amount of short chain fatty acids in cecal contents, the amount of bile acids in small intestinal contents, and fecal excretion of neutral sterols increased logarithmically with increasing DF, while total plasma cholesterol concentration decreased. On the other hand, hepatic CYP7A1 activity, fecal dry weight, and fecal excretion of bile acids increased linearly with increasing DF, while body weight gain decreased. The hypocholesterolemic effect of HACS in OVX-rats appeared to be more effective by heat-moisture treatment.

Ancillary