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Angiotensin-I Converting Enzyme Inhibitory Peptide Derived from Porcine Skeletal Muscle Myosin and Its Antihypertensive Activity in Spontaneously Hypertensive Rats

Authors

  • K. Katayama,

    1. Authors Katayama, Jamhari, Mori, Kawahara, and Muguruma are with Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan. Authors Miake, Kodama, and Sugiyama are with Marudai Food Co. Ltd., Takatsuki, Osaka 569-8577, Japan. Author Kawamura is with Graduate School of Agriculture, Kinki Univ., Nara 3327-204 Japan. Authors Nakayama and Maruyama are with Faculty of Medicine, Univ. of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. Direct inquiries to author Muguruma (E-mail: muguruma@cc.miyazaki-u.ac.jp).
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  • Jamhari,

    1. Authors Katayama, Jamhari, Mori, Kawahara, and Muguruma are with Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan. Authors Miake, Kodama, and Sugiyama are with Marudai Food Co. Ltd., Takatsuki, Osaka 569-8577, Japan. Author Kawamura is with Graduate School of Agriculture, Kinki Univ., Nara 3327-204 Japan. Authors Nakayama and Maruyama are with Faculty of Medicine, Univ. of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. Direct inquiries to author Muguruma (E-mail: muguruma@cc.miyazaki-u.ac.jp).
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  • T. Mori,

    1. Authors Katayama, Jamhari, Mori, Kawahara, and Muguruma are with Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan. Authors Miake, Kodama, and Sugiyama are with Marudai Food Co. Ltd., Takatsuki, Osaka 569-8577, Japan. Author Kawamura is with Graduate School of Agriculture, Kinki Univ., Nara 3327-204 Japan. Authors Nakayama and Maruyama are with Faculty of Medicine, Univ. of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. Direct inquiries to author Muguruma (E-mail: muguruma@cc.miyazaki-u.ac.jp).
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  • S. Kawahara,

    1. Authors Katayama, Jamhari, Mori, Kawahara, and Muguruma are with Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan. Authors Miake, Kodama, and Sugiyama are with Marudai Food Co. Ltd., Takatsuki, Osaka 569-8577, Japan. Author Kawamura is with Graduate School of Agriculture, Kinki Univ., Nara 3327-204 Japan. Authors Nakayama and Maruyama are with Faculty of Medicine, Univ. of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. Direct inquiries to author Muguruma (E-mail: muguruma@cc.miyazaki-u.ac.jp).
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  • K. Miake,

    1. Authors Katayama, Jamhari, Mori, Kawahara, and Muguruma are with Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan. Authors Miake, Kodama, and Sugiyama are with Marudai Food Co. Ltd., Takatsuki, Osaka 569-8577, Japan. Author Kawamura is with Graduate School of Agriculture, Kinki Univ., Nara 3327-204 Japan. Authors Nakayama and Maruyama are with Faculty of Medicine, Univ. of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. Direct inquiries to author Muguruma (E-mail: muguruma@cc.miyazaki-u.ac.jp).
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  • Y. Kodama,

    1. Authors Katayama, Jamhari, Mori, Kawahara, and Muguruma are with Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan. Authors Miake, Kodama, and Sugiyama are with Marudai Food Co. Ltd., Takatsuki, Osaka 569-8577, Japan. Author Kawamura is with Graduate School of Agriculture, Kinki Univ., Nara 3327-204 Japan. Authors Nakayama and Maruyama are with Faculty of Medicine, Univ. of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. Direct inquiries to author Muguruma (E-mail: muguruma@cc.miyazaki-u.ac.jp).
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  • M. Sugiyama,

    1. Authors Katayama, Jamhari, Mori, Kawahara, and Muguruma are with Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan. Authors Miake, Kodama, and Sugiyama are with Marudai Food Co. Ltd., Takatsuki, Osaka 569-8577, Japan. Author Kawamura is with Graduate School of Agriculture, Kinki Univ., Nara 3327-204 Japan. Authors Nakayama and Maruyama are with Faculty of Medicine, Univ. of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. Direct inquiries to author Muguruma (E-mail: muguruma@cc.miyazaki-u.ac.jp).
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  • Y. Kawamura,

    1. Authors Katayama, Jamhari, Mori, Kawahara, and Muguruma are with Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan. Authors Miake, Kodama, and Sugiyama are with Marudai Food Co. Ltd., Takatsuki, Osaka 569-8577, Japan. Author Kawamura is with Graduate School of Agriculture, Kinki Univ., Nara 3327-204 Japan. Authors Nakayama and Maruyama are with Faculty of Medicine, Univ. of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. Direct inquiries to author Muguruma (E-mail: muguruma@cc.miyazaki-u.ac.jp).
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  • T. Nakayama,

    1. Authors Katayama, Jamhari, Mori, Kawahara, and Muguruma are with Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan. Authors Miake, Kodama, and Sugiyama are with Marudai Food Co. Ltd., Takatsuki, Osaka 569-8577, Japan. Author Kawamura is with Graduate School of Agriculture, Kinki Univ., Nara 3327-204 Japan. Authors Nakayama and Maruyama are with Faculty of Medicine, Univ. of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. Direct inquiries to author Muguruma (E-mail: muguruma@cc.miyazaki-u.ac.jp).
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  • M. Maruyama,

    1. Authors Katayama, Jamhari, Mori, Kawahara, and Muguruma are with Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan. Authors Miake, Kodama, and Sugiyama are with Marudai Food Co. Ltd., Takatsuki, Osaka 569-8577, Japan. Author Kawamura is with Graduate School of Agriculture, Kinki Univ., Nara 3327-204 Japan. Authors Nakayama and Maruyama are with Faculty of Medicine, Univ. of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. Direct inquiries to author Muguruma (E-mail: muguruma@cc.miyazaki-u.ac.jp).
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  • M. Muguruma

    1. Authors Katayama, Jamhari, Mori, Kawahara, and Muguruma are with Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan. Authors Miake, Kodama, and Sugiyama are with Marudai Food Co. Ltd., Takatsuki, Osaka 569-8577, Japan. Author Kawamura is with Graduate School of Agriculture, Kinki Univ., Nara 3327-204 Japan. Authors Nakayama and Maruyama are with Faculty of Medicine, Univ. of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. Direct inquiries to author Muguruma (E-mail: muguruma@cc.miyazaki-u.ac.jp).
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Abstract

ABSTRACT:  Crude myosin light chain was extracted from Japanese domestic pork loin and digested with pepsin. Antihypertensive peptide was isolated from this digest as a measure of its inhibitory activity for angiotensin-I converting enzyme (ACE). Through isolation with some chromatographies, a single active fraction was isolated, and it was detected as an octapeptide, Val-Lys-Lys-Val-Leu-Gly-Asn-Pro, from 47th to 54th positions of myosin light chain. The 50% inhibitory concentration of this peptide was 28.5 μM. Kinetic evaluation showed that this peptide was a noncompetitive inhibitor, but it was slowly hydrolyzed by ACE. At the dose of 10 mg/kg, this peptide showed antihypertensive activity after a maximum of 3 h of administration and was estimated as a temporally effective hypotensor.

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