Effects of Banana, Orange, and Apple on Oxidative Stress-Induced Neurotoxicity in PC12 Cells

Authors

  • H.J. Heo,

    1. Authors Heo and Choi are with Div. of Applied Life Science, Inst. of Agriculture and Life Sciences, Gyeongsang Natl. Univ., Jinju, Gyeongnam 660-701, Korea. Authors Choi and Shin are with Graduate School of Biotechnology, Korea Univ., Seoul 136-701, Korea. Author Lee is with Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee Univ., Yongin, Gyeonggi 449-701, Korea. Author Lee is with Dept. of Food Science and Technology, Cornell Univ., Geneva, N.Y. 14456, U.S.A. Direct inquiries to author Lee (E-mail: cyl1@cornell.edu).
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  • S.J. Choi,

    1. Authors Heo and Choi are with Div. of Applied Life Science, Inst. of Agriculture and Life Sciences, Gyeongsang Natl. Univ., Jinju, Gyeongnam 660-701, Korea. Authors Choi and Shin are with Graduate School of Biotechnology, Korea Univ., Seoul 136-701, Korea. Author Lee is with Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee Univ., Yongin, Gyeonggi 449-701, Korea. Author Lee is with Dept. of Food Science and Technology, Cornell Univ., Geneva, N.Y. 14456, U.S.A. Direct inquiries to author Lee (E-mail: cyl1@cornell.edu).
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  • S.-G. Choi,

    1. Authors Heo and Choi are with Div. of Applied Life Science, Inst. of Agriculture and Life Sciences, Gyeongsang Natl. Univ., Jinju, Gyeongnam 660-701, Korea. Authors Choi and Shin are with Graduate School of Biotechnology, Korea Univ., Seoul 136-701, Korea. Author Lee is with Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee Univ., Yongin, Gyeonggi 449-701, Korea. Author Lee is with Dept. of Food Science and Technology, Cornell Univ., Geneva, N.Y. 14456, U.S.A. Direct inquiries to author Lee (E-mail: cyl1@cornell.edu).
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  • D.-H. Shin,

    1. Authors Heo and Choi are with Div. of Applied Life Science, Inst. of Agriculture and Life Sciences, Gyeongsang Natl. Univ., Jinju, Gyeongnam 660-701, Korea. Authors Choi and Shin are with Graduate School of Biotechnology, Korea Univ., Seoul 136-701, Korea. Author Lee is with Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee Univ., Yongin, Gyeonggi 449-701, Korea. Author Lee is with Dept. of Food Science and Technology, Cornell Univ., Geneva, N.Y. 14456, U.S.A. Direct inquiries to author Lee (E-mail: cyl1@cornell.edu).
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  • J.M. Lee,

    1. Authors Heo and Choi are with Div. of Applied Life Science, Inst. of Agriculture and Life Sciences, Gyeongsang Natl. Univ., Jinju, Gyeongnam 660-701, Korea. Authors Choi and Shin are with Graduate School of Biotechnology, Korea Univ., Seoul 136-701, Korea. Author Lee is with Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee Univ., Yongin, Gyeonggi 449-701, Korea. Author Lee is with Dept. of Food Science and Technology, Cornell Univ., Geneva, N.Y. 14456, U.S.A. Direct inquiries to author Lee (E-mail: cyl1@cornell.edu).
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  • C.Y. Lee

    1. Authors Heo and Choi are with Div. of Applied Life Science, Inst. of Agriculture and Life Sciences, Gyeongsang Natl. Univ., Jinju, Gyeongnam 660-701, Korea. Authors Choi and Shin are with Graduate School of Biotechnology, Korea Univ., Seoul 136-701, Korea. Author Lee is with Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee Univ., Yongin, Gyeonggi 449-701, Korea. Author Lee is with Dept. of Food Science and Technology, Cornell Univ., Geneva, N.Y. 14456, U.S.A. Direct inquiries to author Lee (E-mail: cyl1@cornell.edu).
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Abstract

ABSTRACT:  Banana, orange, and apple are the major fruits in Western and Asian diets. In order to find the effects of these fruits, neuron like PC12 cells were exposed to the extracts of these fruits before H2O2 treatment. We found a significant viability of PC12 cells by the MTT reduction test, which indicated that the phenolics of banana, orange, and apple fruits prevented oxidative stress-induced neurotoxicity. Additional tests by lactate dehydrogenase and trypan blue exclusion assays showed that the extracts reduced oxidative stress-induced neuronal cell membrane damage. These results suggest that fresh apples, banana, and orange in our daily diet along with other fruits may protect neuron cells against oxidative stress-induced neurotoxicity and may play an important role in reducing the risk of neurodegenerative disorders such as Alzheimer's disease.

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