Hibiscus sabdariffa L. Extracts Inhibit the Mutagenicity in Microsuspension Assay and the Proliferation of HeLa Cells

  1. V. Olvera-García,
  2. E. Castaño-Tostado,
  3. R. I. Rezendiz-Lopez,
  4. R. Reynoso-Camacho,
  5. E. González de Mejía,
  6. G. Elizondo,
  7. G. Loarca-Piña

Article first published online: 28 MAY 2008

DOI: 10.1111/j.1750-3841.2008.00781.x

Issue

Journal of Food Science

Journal of Food Science

Volume 73, Issue 5, pages T75–T81, June/July 2008

Additional Information

How to Cite

Olvera-García, V., Castaño-Tostado, E., Rezendiz-Lopez, R. I., Reynoso-Camacho, R., González de Mejía, E., Elizondo, G. and Loarca-Piña, G. (2008), Hibiscus sabdariffa L. Extracts Inhibit the Mutagenicity in Microsuspension Assay and the Proliferation of HeLa Cells. Journal of Food Science, 73: T75–T81. doi: 10.1111/j.1750-3841.2008.00781.x

Author Information

  1. Authors Olvera-García, Castaño-Tostado, Rezendiz-Lopez, Reynoso-Camacho, and Loarca-Piña are with PROPAC (Programa de Posgrado en Alimentos del Centro de la República) Univ. Autónoma de Querétaro, Querétaro, Qro., 76010 México. Author González de Mejía is with Dept. of Food Science and Human Nutrition, Univ. of Illinois, Urbana, IL 61801, U.S.A. Author Elizondo is with Programa de Posgrado, Sección Externa de Toxicología, CINVESTAV, Instituto Politécnico Nac., Av. Instituto Politécnico Nac. 2508, Colonia San Pedro Zacatenco, Distrito Federal, 07360 México. Direct inquiries to author Loarca-Piña (E-mail: loarca@uaq.mx or FlaviaLoarca@yahoo.com.mx).

Publication History

  1. Issue published online: 28 MAY 2008
  2. Article first published online: 28 MAY 2008
  3. MS 20070839 Submitted 11/12/2007, Accepted 3/26/2008

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Keywords:

  • 1-NP;
  • HeLa cells;
  • Hibiscus sabdariffa L.;
  • microsuspension assay;
  • protocatechuic acid

ABSTRACT: Hibiscus sabdariffa L. is used as a refreshing beverage and as a traditional medicine. The objective of this study was to determine the in vitro effect of phenolic compounds present in aqueous, ethyl acetate, and chloroform extracts of H. sabdariffa against mutagenicity of 1-nitropyrene (1-NP), and also the antiproliferative effect of these extracts. Inhibition of cell proliferation and DNA fragmentation were tested on transformed human HeLa cells. The hot aqueous extract (HAE) contained 22.27 ± 2.52 mg of protocatechuic acid (PCA) per gram of lyophilized dried extract, and was not statistically different from the cold aqueous or chloroform extracts; the ethyl acetate extract produced the least amount of PCA. The H. sabdariffa extracts inhibited mutagenicity of 1-NP in a dose-response manner. The inhibition rate on HeLa cells of HAE was also dose-dependent. The HAE did not induce DNA fragmentation. The results suggest that H. sabdariffa L. extracts have antimutagenic activity against 1-NP and decrease the proliferation of HeLa cells, probably due to phenolic acid composition.

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