Get access

Superfine Saengshik Improves Liver Protecting Effect Compared with Fine Saengshik in an Animal Model

Authors

  • D.H. Kim,

    1. Author Kim is with Dept. of Environmental Medical Biology and Inst. of Basic Medical Science, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Song is with Dept. of Environmental Medical Biology, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Kang is with Dept. of Plant Biotechnology, School of Bioscience and Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Author Jeong is with Dept. of Molecular and Medical Biotechnology, School of Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Authors Yoon and Y.M. Lee are with the Biotech Research Inst., HDr Co., Ltd., Wonju, Gangwon 220-836, South Korea. Author Chang is with Dept. of Cosmetology, Hanseo Univ., Seosan, Chungnam 356-706, South Korea. Author K.J. Lee is with the Dept. of Environmental Medical Biology and Inst. of Lifelong Health, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Direct inquiries to author K.J. Lee (E-mail: medbio@yonsei.ac.kr).
    Search for more papers by this author
  • S.B. Song,

    1. Author Kim is with Dept. of Environmental Medical Biology and Inst. of Basic Medical Science, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Song is with Dept. of Environmental Medical Biology, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Kang is with Dept. of Plant Biotechnology, School of Bioscience and Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Author Jeong is with Dept. of Molecular and Medical Biotechnology, School of Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Authors Yoon and Y.M. Lee are with the Biotech Research Inst., HDr Co., Ltd., Wonju, Gangwon 220-836, South Korea. Author Chang is with Dept. of Cosmetology, Hanseo Univ., Seosan, Chungnam 356-706, South Korea. Author K.J. Lee is with the Dept. of Environmental Medical Biology and Inst. of Lifelong Health, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Direct inquiries to author K.J. Lee (E-mail: medbio@yonsei.ac.kr).
    Search for more papers by this author
  • W.S. Kang,

    1. Author Kim is with Dept. of Environmental Medical Biology and Inst. of Basic Medical Science, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Song is with Dept. of Environmental Medical Biology, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Kang is with Dept. of Plant Biotechnology, School of Bioscience and Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Author Jeong is with Dept. of Molecular and Medical Biotechnology, School of Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Authors Yoon and Y.M. Lee are with the Biotech Research Inst., HDr Co., Ltd., Wonju, Gangwon 220-836, South Korea. Author Chang is with Dept. of Cosmetology, Hanseo Univ., Seosan, Chungnam 356-706, South Korea. Author K.J. Lee is with the Dept. of Environmental Medical Biology and Inst. of Lifelong Health, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Direct inquiries to author K.J. Lee (E-mail: medbio@yonsei.ac.kr).
    Search for more papers by this author
  • Y.H. Jeong,

    1. Author Kim is with Dept. of Environmental Medical Biology and Inst. of Basic Medical Science, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Song is with Dept. of Environmental Medical Biology, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Kang is with Dept. of Plant Biotechnology, School of Bioscience and Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Author Jeong is with Dept. of Molecular and Medical Biotechnology, School of Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Authors Yoon and Y.M. Lee are with the Biotech Research Inst., HDr Co., Ltd., Wonju, Gangwon 220-836, South Korea. Author Chang is with Dept. of Cosmetology, Hanseo Univ., Seosan, Chungnam 356-706, South Korea. Author K.J. Lee is with the Dept. of Environmental Medical Biology and Inst. of Lifelong Health, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Direct inquiries to author K.J. Lee (E-mail: medbio@yonsei.ac.kr).
    Search for more papers by this author
  • Y.S. Yoon,

    1. Author Kim is with Dept. of Environmental Medical Biology and Inst. of Basic Medical Science, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Song is with Dept. of Environmental Medical Biology, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Kang is with Dept. of Plant Biotechnology, School of Bioscience and Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Author Jeong is with Dept. of Molecular and Medical Biotechnology, School of Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Authors Yoon and Y.M. Lee are with the Biotech Research Inst., HDr Co., Ltd., Wonju, Gangwon 220-836, South Korea. Author Chang is with Dept. of Cosmetology, Hanseo Univ., Seosan, Chungnam 356-706, South Korea. Author K.J. Lee is with the Dept. of Environmental Medical Biology and Inst. of Lifelong Health, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Direct inquiries to author K.J. Lee (E-mail: medbio@yonsei.ac.kr).
    Search for more papers by this author
  • Y.M. Lee,

    1. Author Kim is with Dept. of Environmental Medical Biology and Inst. of Basic Medical Science, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Song is with Dept. of Environmental Medical Biology, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Kang is with Dept. of Plant Biotechnology, School of Bioscience and Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Author Jeong is with Dept. of Molecular and Medical Biotechnology, School of Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Authors Yoon and Y.M. Lee are with the Biotech Research Inst., HDr Co., Ltd., Wonju, Gangwon 220-836, South Korea. Author Chang is with Dept. of Cosmetology, Hanseo Univ., Seosan, Chungnam 356-706, South Korea. Author K.J. Lee is with the Dept. of Environmental Medical Biology and Inst. of Lifelong Health, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Direct inquiries to author K.J. Lee (E-mail: medbio@yonsei.ac.kr).
    Search for more papers by this author
  • B.S. Chang,

    1. Author Kim is with Dept. of Environmental Medical Biology and Inst. of Basic Medical Science, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Song is with Dept. of Environmental Medical Biology, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Kang is with Dept. of Plant Biotechnology, School of Bioscience and Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Author Jeong is with Dept. of Molecular and Medical Biotechnology, School of Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Authors Yoon and Y.M. Lee are with the Biotech Research Inst., HDr Co., Ltd., Wonju, Gangwon 220-836, South Korea. Author Chang is with Dept. of Cosmetology, Hanseo Univ., Seosan, Chungnam 356-706, South Korea. Author K.J. Lee is with the Dept. of Environmental Medical Biology and Inst. of Lifelong Health, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Direct inquiries to author K.J. Lee (E-mail: medbio@yonsei.ac.kr).
    Search for more papers by this author
  • K.J. Lee

    1. Author Kim is with Dept. of Environmental Medical Biology and Inst. of Basic Medical Science, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Song is with Dept. of Environmental Medical Biology, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Author Kang is with Dept. of Plant Biotechnology, School of Bioscience and Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Author Jeong is with Dept. of Molecular and Medical Biotechnology, School of Biotechnology, Kangwon Natl. Univ., Chunchon, Gangwon 200-701, South Korea. Authors Yoon and Y.M. Lee are with the Biotech Research Inst., HDr Co., Ltd., Wonju, Gangwon 220-836, South Korea. Author Chang is with Dept. of Cosmetology, Hanseo Univ., Seosan, Chungnam 356-706, South Korea. Author K.J. Lee is with the Dept. of Environmental Medical Biology and Inst. of Lifelong Health, Wonju College of Medicine, Yonsei Univ., Wonju, Gangwon 220-701, South Korea. Direct inquiries to author K.J. Lee (E-mail: medbio@yonsei.ac.kr).
    Search for more papers by this author

Abstract

ABSTRACT:  This study was performed to compare the effect of liver protection of fine saengshik (FS) and superfine saengshik (SS) and uncooked and powdered grains and vegetables, produced by the different mill technique on the acute hepatotoxicity induced by CCl4 in mouse. As the result of particle size distribution in number, particles included under 0.955 μm dia were 7.02% and 68.92% respectively. Hematological and serological examination showed that AST (P < 0.05) and ALT (P < 0.05) of SS + CCl4 group decreased significantly compared with those of FS + CCl4 group. On the examination of antioxidant effect, water extract of SS showed a higher superoxide dismutase (SOD)-like activity on the condition of the HX/XOD system than that of FS (P < 0.001). Also, the glutathione peroxidase (P < 0.01) and glutathione reductase (P < 0.05) activities in liver showed a significant difference between FS + CCl4 and SS + CCl4 groups. On the histological observation of liver, SS + CCl4 group showed a mild reversible hepatocytic change and infiltration of inflammatory cells around the central veins, whereas FS + CCl4 group showed severe agglutination necrosis by CCl4 toxicity. These results suggest that superfine saengshik significantly improves liver protection effect compared with fine saengshik; its major mechanism is supposed to be the improved antioxidant effect of saengshik by reduced size of particles.

Ancillary