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Antistaphylococcal Effect of Enterocin AS-48 in Bakery Ingredients of Vegetable Origin, Alone and in Combination with Selected Antimicrobials

Authors

  • Pilar Martinez Viedma,

    1. Authors are with Área de Microbiología, Depto. de Ciencias de la Salud, Facultad de Ciencias Experimentales, Univ. de Jaén, 23071-Jaén, Spain. Direct inquiries to author Gálvez (E-mail: agalvez@ujaen.es).
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  • Hikmate Abriouel,

    1. Authors are with Área de Microbiología, Depto. de Ciencias de la Salud, Facultad de Ciencias Experimentales, Univ. de Jaén, 23071-Jaén, Spain. Direct inquiries to author Gálvez (E-mail: agalvez@ujaen.es).
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  • Nabil Ben Omar,

    1. Authors are with Área de Microbiología, Depto. de Ciencias de la Salud, Facultad de Ciencias Experimentales, Univ. de Jaén, 23071-Jaén, Spain. Direct inquiries to author Gálvez (E-mail: agalvez@ujaen.es).
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  • Rosario Lucas López,

    1. Authors are with Área de Microbiología, Depto. de Ciencias de la Salud, Facultad de Ciencias Experimentales, Univ. de Jaén, 23071-Jaén, Spain. Direct inquiries to author Gálvez (E-mail: agalvez@ujaen.es).
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  • Antonio Gálvez

    1. Authors are with Área de Microbiología, Depto. de Ciencias de la Salud, Facultad de Ciencias Experimentales, Univ. de Jaén, 23071-Jaén, Spain. Direct inquiries to author Gálvez (E-mail: agalvez@ujaen.es).
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Abstract

ABSTRACT:  The inhibitory effect of enterocin AS-48 against Staphylococcus aureus was investigated in various types of bakery ingredients. Antibacterial activity greatly depended on the food substrate, ranging from complete inactivation of S. aureus in liquid caramel (in which the bacterium survived poorly) to no significant inhibition (as in vanilla or chocolate creams). Significant reductions of viable counts in the range of 1.8 to 2.7 log units (P < 0.05) were achieved in substrates like pumpkin confiture or diluted almond cream stored at temperatures of 10 or 22 °C. Given the very low activity detected in chocolate substrates, enterocin AS-48 was tested in combination with other antimicrobials. Bactericidal activity increased markedly for the combinations of AS-48 and 0.1% eugenol (v/v), 0.5% 2-nitropropanol (v/v), or 3% Nisaplin (w/v). Enterocin AS-48 could be applied in combination with other antimicrobials for preservation of bakery ingredients against S. aureus.

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