Acrylamide–Sepiolite Based Composite Hydrogels for Immobilization of Invertase
Version of Record online: 20 AUG 2009
© 2009 Institute of Food Technologists®
Journal of Food Science
Volume 74, Issue 7, pages N45–N49, September 2009
How to Cite
Öztop, H. N., Hepokur, C. and Saraydin, D. (2009), Acrylamide–Sepiolite Based Composite Hydrogels for Immobilization of Invertase. Journal of Food Science, 74: N45–N49. doi: 10.1111/j.1750-3841.2009.01302.x
- Issue online: 1 SEP 2009
- Version of Record online: 20 AUG 2009
- MS 20090343 Submitted 4/17/2009, Accepted 7/2/2009.
ABSTRACT: Novel composite hydrogels, poly(acrylamide)–sepiolite (PAS), poly(acrylamide/acrylic acid)–sepiolite (PAAS), and poly(acrylamide/itaconic acid)–sepiolite (PAIS) were prepared and used for the immobilization of invertase. The parameters of equilibrium swelling, diffusional exponent, and diffusion coefficient of these hydrogels were calculated from swelling experiments. Invertase was immobilized onto PAS, PAAS, and PAIS and immobilized invertases (PASI, PAASI, and PAISI) were prepared. Optimum pH values for free invertase, PASI, PAASI, and PAISI are found to be 5, 5.5, 4.5, and 6, respectively, and the optimum temperatures were 30, 50, 50, and 35 °C for free invertase PASI, PAASI, and PAISI. It was found that Km values of free invertase, PASI, PAASI, and PAISI were 11.3, 41.0, 94.5, and 56.0 mM, respectively. Vmax values were 2 μmol/min for free invertase, 8.10 μmol/min for PASI, 1.30 μmol/min for PAASI, and 0.42 μmol/min for PAISI, respectively. The invertase immobilized hydrogels showed excellent, temperature, storage, and operational stability.