Anti-inflammatory Effects of Limonene from Yuzu (Citrus junos Tanaka) Essential Oil on Eosinophils
Article first published online: 11 MAR 2010
© 2010 Institute of Food Technologists®
Journal of Food Science
Volume 75, Issue 3, pages H87–H92, April 2010
How to Cite
Hirota, R., Roger, N. N., Nakamura, H., Song, H.-S., Sawamura, M. and Suganuma, N. (2010), Anti-inflammatory Effects of Limonene from Yuzu (Citrus junos Tanaka) Essential Oil on Eosinophils. Journal of Food Science, 75: H87–H92. doi: 10.1111/j.1750-3841.2010.01541.x
- Issue published online: 5 APR 2010
- Article first published online: 11 MAR 2010
- MS 20090713 Submitted 7/26/2009, Accepted 1/10/2010.
- monocyte chemoattractant protein-1 (MCP-1);
- nuclear factor (NF) kappa B;
- p38 mitogen-activated protein kinase (MAPK);
- reactive oxygen species (ROS);
- yuzu (Citrus junos Tanaka) essential oil
ABSTRACT: Yuzu (Citrus junos Tanaka) has been used as a traditional medicine in Japan. We investigated in vitro anti-inflammatory effects of limonene from yuzu peel on human eosinophilic leukemia HL-60 clone 15 cells. To examine anti-inflammatory effects of limonene on the cells, we measured the level of reactive oxygen species (ROS), monocyte chemoattractant protein-1 (MCP-1), nuclear factor (NF) kappa B, and p38 mitogen-activated protein kinase (MAPK). We found that low concentration of limonene (7.34 mmol/L) inhibited the production of ROS for eotaxin-stimulated HL-60 clone 15 cells. 14.68 mmol/L concentration of limonene diminished MCP-1 production via NF-kappa B activation comparable to the addition of the proteasomal inhibitor MG132. In addition, it inhibited cell chemotaxis in a p38 MAPK dependent manner similar to the adding of SB203580. These results suggest that limonene may have potential anti-inflammatory efficacy for the treatment of bronchial asthma by inhibiting cytokines, ROS production, and inactivating eosinophil migration.