Effects of Vitamin D3 on Expression of Tumor Necrosis Factor-α and Chemokines by Monocytes

Authors

  • Yu-Ting Kuo,

    1. Authors Y.-T. Kuo, C.-H. Kuo, Lam, Chu, Wang, and Hung are with Dept. of Pediatrics, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Author Huang is with Dept. of Pediatric, Yuan's General Hospital, Kaoshiung, Taiwan. Author Y.-T. Kuo is also with Dept. of Emergency, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo, Wang, and Hung are also with Dept. of Pediatrics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo and Hung are also with Graduate Inst. of Medicine, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Author Hung is also with Faculty of Pediatrics, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Direct inquiries to author Hung (E-mail: pedhung@gmail.com).
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  • Chang-Hung Kuo,

    1. Authors Y.-T. Kuo, C.-H. Kuo, Lam, Chu, Wang, and Hung are with Dept. of Pediatrics, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Author Huang is with Dept. of Pediatric, Yuan's General Hospital, Kaoshiung, Taiwan. Author Y.-T. Kuo is also with Dept. of Emergency, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo, Wang, and Hung are also with Dept. of Pediatrics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo and Hung are also with Graduate Inst. of Medicine, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Author Hung is also with Faculty of Pediatrics, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Direct inquiries to author Hung (E-mail: pedhung@gmail.com).
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  • Ka-Pan Lam,

    1. Authors Y.-T. Kuo, C.-H. Kuo, Lam, Chu, Wang, and Hung are with Dept. of Pediatrics, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Author Huang is with Dept. of Pediatric, Yuan's General Hospital, Kaoshiung, Taiwan. Author Y.-T. Kuo is also with Dept. of Emergency, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo, Wang, and Hung are also with Dept. of Pediatrics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo and Hung are also with Graduate Inst. of Medicine, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Author Hung is also with Faculty of Pediatrics, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Direct inquiries to author Hung (E-mail: pedhung@gmail.com).
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  • Yu-Te Chu,

    1. Authors Y.-T. Kuo, C.-H. Kuo, Lam, Chu, Wang, and Hung are with Dept. of Pediatrics, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Author Huang is with Dept. of Pediatric, Yuan's General Hospital, Kaoshiung, Taiwan. Author Y.-T. Kuo is also with Dept. of Emergency, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo, Wang, and Hung are also with Dept. of Pediatrics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo and Hung are also with Graduate Inst. of Medicine, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Author Hung is also with Faculty of Pediatrics, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Direct inquiries to author Hung (E-mail: pedhung@gmail.com).
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  • Wei-Li Wang,

    1. Authors Y.-T. Kuo, C.-H. Kuo, Lam, Chu, Wang, and Hung are with Dept. of Pediatrics, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Author Huang is with Dept. of Pediatric, Yuan's General Hospital, Kaoshiung, Taiwan. Author Y.-T. Kuo is also with Dept. of Emergency, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo, Wang, and Hung are also with Dept. of Pediatrics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo and Hung are also with Graduate Inst. of Medicine, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Author Hung is also with Faculty of Pediatrics, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Direct inquiries to author Hung (E-mail: pedhung@gmail.com).
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  • Ching-Hua Huang,

    1. Authors Y.-T. Kuo, C.-H. Kuo, Lam, Chu, Wang, and Hung are with Dept. of Pediatrics, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Author Huang is with Dept. of Pediatric, Yuan's General Hospital, Kaoshiung, Taiwan. Author Y.-T. Kuo is also with Dept. of Emergency, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo, Wang, and Hung are also with Dept. of Pediatrics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo and Hung are also with Graduate Inst. of Medicine, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Author Hung is also with Faculty of Pediatrics, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Direct inquiries to author Hung (E-mail: pedhung@gmail.com).
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  • Chih-Hsing Hung

    1. Authors Y.-T. Kuo, C.-H. Kuo, Lam, Chu, Wang, and Hung are with Dept. of Pediatrics, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Author Huang is with Dept. of Pediatric, Yuan's General Hospital, Kaoshiung, Taiwan. Author Y.-T. Kuo is also with Dept. of Emergency, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo, Wang, and Hung are also with Dept. of Pediatrics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo and Hung are also with Graduate Inst. of Medicine, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Author Hung is also with Faculty of Pediatrics, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Direct inquiries to author Hung (E-mail: pedhung@gmail.com).
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  • Y.-T. Kuo and C.-H. Kuo contributed equally to the manuscript.

Abstract

Abstract:  The association between vitamin D deficiency and asthma epidemic has been recognized. Tumor necrosis factor (TNF)-α and chemokines play important roles in pathogenesis of asthma. However, whether vitamin D has immunoregulatory function on TNF-α and chemokines expression in human monocytes is still unknown. The human monocytic cell line, THP-1 cells and human primary monocytes were pretreated with various concentration of 1α,25-(OH)2D3 for 2 h before stimulation with lipopolysaccharide (LPS). Supernatants were collected 24 or 48 h after LPS stimulation. The levels of TNF-α, interferon-inducible protein 10 (IP-10)/CXCL10 (the Th1-related chemokine), macrophage-derived chemokine (MDC)/ CCL22 (the Th2-related chemokine), and interleukin 8 (IL-8)/CXCL8 (the neutrophil chemoattractant) were measured by ELISA. 1α,25-(OH)2D3 could significantly suppress TNF-α and IP-10 expression in LPS-stimulated THP-1 and human primary monocytes. However, 1α,25-(OH)2D3, especially in higher concentration, could significantly enhance MDC expression. 1α,25-(OH)2D3 had no significant effects on IL-8 expression. We found 1α,25-(OH)2D3 could significantly suppress TNF-α and Th1-related chemokine IP-10, which both play important roles in pathogenesis of severe refractory asthma and autoimmune diseases. However, 1α,25-(OH)2D3 enhanced Th2-related chemokine MDC, which may result in Th2 inflammatory cell recruitment and thus adversely affect asthmatic patients. Although vitamin D has potential utility in the treatment of asthma and autoimmune diseases, excessive use of vitamin D may not be suitable in patients with Th2 allergic diseases.

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