Get access

Distribution of Stable Free Radicals among Amino Acids of Isolated Soy Proteins

Authors

  • Qingxin Lei,

    1. Authors Lei, Liebold, and Boatright are with Dept. of Animal and Food Sciences, Univ. of Kentucky, 412 W.P. Garrigus Building, Lexington, KY 40546-0215, U.S.A. Author Shah Jahan is with Dept. of Physics, Univ. of Memphis, 216 Manning Hall, Memphis, TN 38512, U.S.A. Direct inquiries to author Boatright (E-mail: wlboat1@uky.edu).
    Search for more papers by this author
  • Christopher M. Liebold,

    1. Authors Lei, Liebold, and Boatright are with Dept. of Animal and Food Sciences, Univ. of Kentucky, 412 W.P. Garrigus Building, Lexington, KY 40546-0215, U.S.A. Author Shah Jahan is with Dept. of Physics, Univ. of Memphis, 216 Manning Hall, Memphis, TN 38512, U.S.A. Direct inquiries to author Boatright (E-mail: wlboat1@uky.edu).
    Search for more papers by this author
  • William L. Boatright,

    1. Authors Lei, Liebold, and Boatright are with Dept. of Animal and Food Sciences, Univ. of Kentucky, 412 W.P. Garrigus Building, Lexington, KY 40546-0215, U.S.A. Author Shah Jahan is with Dept. of Physics, Univ. of Memphis, 216 Manning Hall, Memphis, TN 38512, U.S.A. Direct inquiries to author Boatright (E-mail: wlboat1@uky.edu).
    Search for more papers by this author
  • M. Shah Jahan

    1. Authors Lei, Liebold, and Boatright are with Dept. of Animal and Food Sciences, Univ. of Kentucky, 412 W.P. Garrigus Building, Lexington, KY 40546-0215, U.S.A. Author Shah Jahan is with Dept. of Physics, Univ. of Memphis, 216 Manning Hall, Memphis, TN 38512, U.S.A. Direct inquiries to author Boatright (E-mail: wlboat1@uky.edu).
    Search for more papers by this author

Abstract

Abstract:  Application of deuterium sulfide to powdered isolated soy proteins (ISP) was used to quench stable free radicals and produce a single deuterium label on amino acids where free radicals reside. The deuterium labels rendered increases of isotope ratio for the specific ions of radical-bearing amino acids. Isotope ratio measurements were achieved by gas chromatography/mass spectrometry (GC/MS) analyses after the amino acids were released by acidic hydrolysis and converted to volatile derivatives with propyl chloroformate. The isotope enrichment data showed the stable free radicals were located on Ala, Gly, Leu, Ile, Asx (Asp+Asn), Glx (Glu+Gln), and Trp but not on Val, Pro, Met, Phe, Lys, and His. Due to the low abundance of Ser, Thr, and Cys derivatives and the impossibility to accurately measure their isotope ratios, the radical bearing status for these amino acids remained undetermined even though their derivatives were positively identified from ISP hydrolysates. The relative isotope enrichment for radical-bearing amino acids Ala, Gly, Leu, Ile, Asx (Asp+Asn), Glx (Glu+Gln), and Trp were 8.67%, 2.96%, 2.90%, 3.94%, 6.03%, 3.91%, and 21.48%, respectively. Isotope ratio increase for Tyr was also observed but further investigation revealed such increase was mainly from nonspecific deuterium–hydrogen exchange not free radical quenching. The results obtained from the present study provide important information for a better understanding of the mechanisms of free radical formation and stabilization in “dry” ISP.

Ancillary