Cloud-Point Extraction and Reversed-Phase High-Performance Liquid Chromatography for the Determination of Synthetic Phenolic Antioxidants in Edible Oils
Version of Record online: 29 NOV 2010
© 2010 Institute of Food Technologists®
Journal of Food Science
Volume 76, Issue 1, pages C98–C103, January/February 2011
How to Cite
Chen, M., Xia, Q., Liu, M. and Yang, Y. (2011), Cloud-Point Extraction and Reversed-Phase High-Performance Liquid Chromatography for the Determination of Synthetic Phenolic Antioxidants in Edible Oils. Journal of Food Science, 76: C98–C103. doi: 10.1111/j.1750-3841.2010.01914.x
- Issue online: 13 JAN 2011
- Version of Record online: 29 NOV 2010
- MS 20100744 Submitted 7/1/2010, Accepted 9/27/2010.
- cloud-point extraction;
- edible oils;
- high-performance liquid chromatography;
- synthetic phenolic antioxidants;
- Triton X-114
Abstract: A cloud-point extraction (CPE) method using Triton X-114 (TX-114) nonionic surfactant was developed for the extraction and preconcentration of propyl gallate (PG), tertiary butyl hydroquinone (TBHQ), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) from edible oils. The optimum conditions of CPE were 2.5% (v/v) TX-114, 0.5% (w/v) NaCl and 40 min equilibration time at 50 °C. The surfactant-rich phase was then analyzed by reversed-phase high-performance liquid chromatography with ultraviolet detection at 280 nm, using a gradient mobile phase consisting of methanol and 1.5% (v/v) acetic acid. Under the studied conditions, 4 synthetic phenolic antioxidants (SPAs) were successfully separated within 24 min. The limits of detection (LOD) were 1.9 ng mL−1 for PG, 11 ng mL−1 for TBHQ, 2.3 ng mL−1 for BHA, and 5.9 ng mL−1 for BHT. Recoveries of the SPAs spiked into edible oil were in the range 81% to 88%. The CPE method was shown to be potentially useful for the preconcentration of the target analytes, with a preconcentration factor of 14. Moreover, the method is simple, has high sensitivity, consumes much less solvent than traditional methods, and is environment-friendly.
Practical Application: The method established in this article uses less organic solvent to extract SPAs from edible oils; it is simple, highly sensitive and results in no pollution to the environment.