Production of L-Arabinose from Corn Hull Arabinoxylan by Arthrobacter aurescens MK5 α-L-Arabinofuranosidase
Article first published online: 1 MAR 2011
© 2011 Institute of Food Technologists®
Journal of Food Science
Volume 76, Issue 2, pages C231–C235, March 2011
How to Cite
Kurakake, M., Takao, J., Asano, O., Tanimoto, H. and Komaki, T. (2011), Production of L-Arabinose from Corn Hull Arabinoxylan by Arthrobacter aurescens MK5 α-L-Arabinofuranosidase. Journal of Food Science, 76: C231–C235. doi: 10.1111/j.1750-3841.2010.02028.x
- Issue published online: 1 MAR 2011
- Article first published online: 1 MAR 2011
- MS 20100781 Submitted 7/13/2010, Accepted 12/4/2010.
- corn hull
Abstract: Arabinoxylans, which are comprised of a xylan backbone to which are attached glycosyl units that are primarily L-arabinofuranosyl units, are ubiquitous among plant species where it is a constituent of the cell wall. Arabinoxylan has attracted much attention as a potential biomass resource and L-arabinose has recently been reported to possess functional properties that are effective in the treatment of diabetes. Here, we report an α-L-arabinofuranohydrolase, isolated from the soil microbe Arthrobacter aurescens strain MK5, effective in releasing L-arabinose from corn hull arabinoxylan. When A. aurescens strain MK5 was grown in a liquid medium, corn hull arabinoxylan, which has a higher arabinose content (Ara/Xyl = 0.6) than oat spelts xylan (Ara/Xyl = 0.12), induced more efficient arabinoxylan hydrolase production. Analysis of enzyme activity in the culture broth revealed that arabinoxylan hydrolase activity was high, and α-L-arabinofuranosidase and β-xylosidase activities were low. The optimum pH of the MK5 arabinoxylan hydrolase at 40 °C was around 7 and enzyme activity was relatively stable at an alkaline pH up to 9.5. The optimum temperature at pH 7 was around 50 °C and enzyme activity was stable under 50 °C. During the hydrolysis of corn hull arabinoxylan, only L-arabinose was released and 45.1% maximum sugar recovery was achieved. The A. aurescens MK5 enzyme was a typical arabinoxylan α-L-arabinofuranohydrolase and was most effective at releasing L-arabinose from corn hull arabinoxylan, which has a high arabinose content. This enzyme may have important industrial applications.