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Modulation of Th1/Th2 Balance by Lactobacillus Strains Isolated from Kimchi via Stimulation of Macrophage Cell Line J774A.1 In Vitro

Authors

  • Tae Joon Won,

    1. Authors Won, Song, Lim, Oh, Lee, and Hwang are with Immune Modulation Lab. and author Min is with College of Pharmacy, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. Author Kim is with CJ Foods R&D Center, CJ CheilJedang Corp., 636 Guro-dong, Guro-gu, Seoul, Republic of Korea. Author E.S. Park is with Dept. of Pathology, College of Medicine, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea. Author S.Y. Park is with Dept. of Nanobiomedical Science, College of Advanced Science, Dankook Univ., Chungnam 330-714, Republic of Korea. Direct inquiries to authors S.Y. Park (E-mail: soypark23@dankook.ac.kr) or K.W. Hwang (E-mail: khwang@cau.ac.kr).
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  • Bongjoon Kim,

    1. Authors Won, Song, Lim, Oh, Lee, and Hwang are with Immune Modulation Lab. and author Min is with College of Pharmacy, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. Author Kim is with CJ Foods R&D Center, CJ CheilJedang Corp., 636 Guro-dong, Guro-gu, Seoul, Republic of Korea. Author E.S. Park is with Dept. of Pathology, College of Medicine, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea. Author S.Y. Park is with Dept. of Nanobiomedical Science, College of Advanced Science, Dankook Univ., Chungnam 330-714, Republic of Korea. Direct inquiries to authors S.Y. Park (E-mail: soypark23@dankook.ac.kr) or K.W. Hwang (E-mail: khwang@cau.ac.kr).
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  • Dong Sup Song,

    1. Authors Won, Song, Lim, Oh, Lee, and Hwang are with Immune Modulation Lab. and author Min is with College of Pharmacy, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. Author Kim is with CJ Foods R&D Center, CJ CheilJedang Corp., 636 Guro-dong, Guro-gu, Seoul, Republic of Korea. Author E.S. Park is with Dept. of Pathology, College of Medicine, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea. Author S.Y. Park is with Dept. of Nanobiomedical Science, College of Advanced Science, Dankook Univ., Chungnam 330-714, Republic of Korea. Direct inquiries to authors S.Y. Park (E-mail: soypark23@dankook.ac.kr) or K.W. Hwang (E-mail: khwang@cau.ac.kr).
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  • Young Tae Lim,

    1. Authors Won, Song, Lim, Oh, Lee, and Hwang are with Immune Modulation Lab. and author Min is with College of Pharmacy, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. Author Kim is with CJ Foods R&D Center, CJ CheilJedang Corp., 636 Guro-dong, Guro-gu, Seoul, Republic of Korea. Author E.S. Park is with Dept. of Pathology, College of Medicine, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea. Author S.Y. Park is with Dept. of Nanobiomedical Science, College of Advanced Science, Dankook Univ., Chungnam 330-714, Republic of Korea. Direct inquiries to authors S.Y. Park (E-mail: soypark23@dankook.ac.kr) or K.W. Hwang (E-mail: khwang@cau.ac.kr).
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  • Eun Seul Oh,

    1. Authors Won, Song, Lim, Oh, Lee, and Hwang are with Immune Modulation Lab. and author Min is with College of Pharmacy, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. Author Kim is with CJ Foods R&D Center, CJ CheilJedang Corp., 636 Guro-dong, Guro-gu, Seoul, Republic of Korea. Author E.S. Park is with Dept. of Pathology, College of Medicine, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea. Author S.Y. Park is with Dept. of Nanobiomedical Science, College of Advanced Science, Dankook Univ., Chungnam 330-714, Republic of Korea. Direct inquiries to authors S.Y. Park (E-mail: soypark23@dankook.ac.kr) or K.W. Hwang (E-mail: khwang@cau.ac.kr).
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  • Do Ik Lee,

    1. Authors Won, Song, Lim, Oh, Lee, and Hwang are with Immune Modulation Lab. and author Min is with College of Pharmacy, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. Author Kim is with CJ Foods R&D Center, CJ CheilJedang Corp., 636 Guro-dong, Guro-gu, Seoul, Republic of Korea. Author E.S. Park is with Dept. of Pathology, College of Medicine, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea. Author S.Y. Park is with Dept. of Nanobiomedical Science, College of Advanced Science, Dankook Univ., Chungnam 330-714, Republic of Korea. Direct inquiries to authors S.Y. Park (E-mail: soypark23@dankook.ac.kr) or K.W. Hwang (E-mail: khwang@cau.ac.kr).
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  • Eon Sub Park,

    1. Authors Won, Song, Lim, Oh, Lee, and Hwang are with Immune Modulation Lab. and author Min is with College of Pharmacy, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. Author Kim is with CJ Foods R&D Center, CJ CheilJedang Corp., 636 Guro-dong, Guro-gu, Seoul, Republic of Korea. Author E.S. Park is with Dept. of Pathology, College of Medicine, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea. Author S.Y. Park is with Dept. of Nanobiomedical Science, College of Advanced Science, Dankook Univ., Chungnam 330-714, Republic of Korea. Direct inquiries to authors S.Y. Park (E-mail: soypark23@dankook.ac.kr) or K.W. Hwang (E-mail: khwang@cau.ac.kr).
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  • Hyeyoung Min,

    1. Authors Won, Song, Lim, Oh, Lee, and Hwang are with Immune Modulation Lab. and author Min is with College of Pharmacy, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. Author Kim is with CJ Foods R&D Center, CJ CheilJedang Corp., 636 Guro-dong, Guro-gu, Seoul, Republic of Korea. Author E.S. Park is with Dept. of Pathology, College of Medicine, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea. Author S.Y. Park is with Dept. of Nanobiomedical Science, College of Advanced Science, Dankook Univ., Chungnam 330-714, Republic of Korea. Direct inquiries to authors S.Y. Park (E-mail: soypark23@dankook.ac.kr) or K.W. Hwang (E-mail: khwang@cau.ac.kr).
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  • So-Young Park,

    1. Authors Won, Song, Lim, Oh, Lee, and Hwang are with Immune Modulation Lab. and author Min is with College of Pharmacy, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. Author Kim is with CJ Foods R&D Center, CJ CheilJedang Corp., 636 Guro-dong, Guro-gu, Seoul, Republic of Korea. Author E.S. Park is with Dept. of Pathology, College of Medicine, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea. Author S.Y. Park is with Dept. of Nanobiomedical Science, College of Advanced Science, Dankook Univ., Chungnam 330-714, Republic of Korea. Direct inquiries to authors S.Y. Park (E-mail: soypark23@dankook.ac.kr) or K.W. Hwang (E-mail: khwang@cau.ac.kr).
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  • Kwang Woo Hwang

    1. Authors Won, Song, Lim, Oh, Lee, and Hwang are with Immune Modulation Lab. and author Min is with College of Pharmacy, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea. Author Kim is with CJ Foods R&D Center, CJ CheilJedang Corp., 636 Guro-dong, Guro-gu, Seoul, Republic of Korea. Author E.S. Park is with Dept. of Pathology, College of Medicine, Chung-Ang Univ., 221 Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea. Author S.Y. Park is with Dept. of Nanobiomedical Science, College of Advanced Science, Dankook Univ., Chungnam 330-714, Republic of Korea. Direct inquiries to authors S.Y. Park (E-mail: soypark23@dankook.ac.kr) or K.W. Hwang (E-mail: khwang@cau.ac.kr).
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  • Tae Joon Won and Bongjoon Kim contributed equally to this study.

Abstract

Abstract:  Lactobacilli isolated from Kimchi, a Korean traditional food, were tested for their capacity to modulate the T helper (Th) 1/Th2 balance. Ovalbumin (OVA)-sensitized mouse splenocytes were cultured with 26 strains of lactobacilli; the highest IL-12 induction and lowest IL-4 production were then observed in 4 strains, including Lactobacillus plantarum CJLP55, CJLP56, CJLP133, and CJLP136. These strains produced a larger amount of IL-12, which enhances differentiation and activation of Th1 cells, in macrophage cell-lines more than positive control strains L. casei KCTC 3109T and L. rhamnosus GG, although they also induced production of IL-10, which is a suppressor of IL-12. Indeed, CJLP133-stimulated macrophages induced production of more Th1 cytokine IFN-γ and less Th2 cytokine IL-4 than KCTC 3109T and GG in co-cultivation with T cells. These findings suggest that lactobacilli from Kimchi may modulate the Th1/Th2 balance via macrophage activation in the hypersensitive reaction caused by Th2 cells.

Practical Application:  Allergic reactions including asthma and atopy are caused by predominance of Th2 response over Th1 response. Lactobacilli isolated from fermented foods such as yogurt, cheese, and Kimchi showed health-promoting activities. The present study indicated that several lactobacilli strains from Kimchi may reduce allergic reactions through macrophage-mediated induction of Th1 response.

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