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Isolation and Characterization of Lecithin from Squid (Todarodes pacificus) Viscera Deoiled by Supercritical Carbon Dioxide Extraction

Authors

  • Md. Salim Uddin,

    1. Authors Uddin and Chun are with Dept. of Food Science and Technology, Pukyong Natl. Univ., 599-1 Daeyeon-3dong, Nam-Gu, Busan 608-737, Republic of Korea. Author Kishimura is with Lab. of Marine Products and Food Science, Research Faculty of Fisheries Sciences, Hokkaido Univ., Hakodate, Hokkaido 041-8611, Japan. Direct inquiries to author Chun (E-mail: bschun@pknu.ac.kr).
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  • Hideki Kishimura,

    1. Authors Uddin and Chun are with Dept. of Food Science and Technology, Pukyong Natl. Univ., 599-1 Daeyeon-3dong, Nam-Gu, Busan 608-737, Republic of Korea. Author Kishimura is with Lab. of Marine Products and Food Science, Research Faculty of Fisheries Sciences, Hokkaido Univ., Hakodate, Hokkaido 041-8611, Japan. Direct inquiries to author Chun (E-mail: bschun@pknu.ac.kr).
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  • Byung-Soo Chun

    1. Authors Uddin and Chun are with Dept. of Food Science and Technology, Pukyong Natl. Univ., 599-1 Daeyeon-3dong, Nam-Gu, Busan 608-737, Republic of Korea. Author Kishimura is with Lab. of Marine Products and Food Science, Research Faculty of Fisheries Sciences, Hokkaido Univ., Hakodate, Hokkaido 041-8611, Japan. Direct inquiries to author Chun (E-mail: bschun@pknu.ac.kr).
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Abstract

Abstract:  Marine lecithin was isolated and characterized from squid (Todarodes pacificus) viscera residues deoiled by supercritical carbon dioxide (SC-CO2) extraction. SC-CO2 extraction was carried out to extract the oil from squid viscera at different temperatures (35 to 45 °C) and pressures (15 to 25 MPa). The extraction yield was higher at highest temperature and pressure. The major phospholipids of squid viscera lecithin were quantified by high-performance liquid chromatography (HPLC). Phosphatidylcholine (PC; 80.5%± 0.7%) and phosphatidylethanolamine (PE; 13.2%± 0.2%) were the main phospholipids. Thin layer chromatography (TLC) was performed to purify the individual phospholipids. The fatty acid compositions of lecithin, PC and PE were analyzed by gas chromatography (GC). A significant amount of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were present in both phospholipids of PC and PE. Emulsions of lecithin in water were prepared through the use of a homogenizer. The oxidative stability of squid viscera lecithin was high in spite of its high concentration of long-chain polyunsaturated fatty acids.

Practical Application:  Squid viscera are discarded as a waste by fish processing industry. Since lecithin from squid viscera contains higher amounts of polyunsaturated fatty acids, it may have promising effect to use in food, pharmaceutical, and cosmetic industries.

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