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Antiproliferative Activity of Fucoidan Was Associated with the Induction of Apoptosis and Autophagy in AGS Human Gastric Cancer Cells

Authors

  • Hyun Soo Park,

    1. Authors Park and N.D. Kim are with Dept. of Pharmacy, Pusan Natl. Univ., Busan, Republic of Korea. Author G.Y. Kim is with Dept. of Marine Life Sciences, Jeju Natl. Univ., Jeju, Republic of Korea. Author Nam is with Faculty of Food Science and Biotechnology, Pukyong Natl. Univ., Busan, Republic of Korea. Author Choi is with Dept. of Biochemistry, College of Oriental Medicine and Research Inst. of Oriental Medicine and Dept. of Biomaterial Control, Graduate School and Blue-Bio Industry Regional Innovation Center, Dongeui Univ., Busan, Republic of Korea. Direct inquiries to author Choi (E-mail: choiyh@deu.ac.kr).
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  • Gi-Young Kim,

    1. Authors Park and N.D. Kim are with Dept. of Pharmacy, Pusan Natl. Univ., Busan, Republic of Korea. Author G.Y. Kim is with Dept. of Marine Life Sciences, Jeju Natl. Univ., Jeju, Republic of Korea. Author Nam is with Faculty of Food Science and Biotechnology, Pukyong Natl. Univ., Busan, Republic of Korea. Author Choi is with Dept. of Biochemistry, College of Oriental Medicine and Research Inst. of Oriental Medicine and Dept. of Biomaterial Control, Graduate School and Blue-Bio Industry Regional Innovation Center, Dongeui Univ., Busan, Republic of Korea. Direct inquiries to author Choi (E-mail: choiyh@deu.ac.kr).
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  • Taek-Jeong Nam,

    1. Authors Park and N.D. Kim are with Dept. of Pharmacy, Pusan Natl. Univ., Busan, Republic of Korea. Author G.Y. Kim is with Dept. of Marine Life Sciences, Jeju Natl. Univ., Jeju, Republic of Korea. Author Nam is with Faculty of Food Science and Biotechnology, Pukyong Natl. Univ., Busan, Republic of Korea. Author Choi is with Dept. of Biochemistry, College of Oriental Medicine and Research Inst. of Oriental Medicine and Dept. of Biomaterial Control, Graduate School and Blue-Bio Industry Regional Innovation Center, Dongeui Univ., Busan, Republic of Korea. Direct inquiries to author Choi (E-mail: choiyh@deu.ac.kr).
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  • Nam Deuk Kim,

    1. Authors Park and N.D. Kim are with Dept. of Pharmacy, Pusan Natl. Univ., Busan, Republic of Korea. Author G.Y. Kim is with Dept. of Marine Life Sciences, Jeju Natl. Univ., Jeju, Republic of Korea. Author Nam is with Faculty of Food Science and Biotechnology, Pukyong Natl. Univ., Busan, Republic of Korea. Author Choi is with Dept. of Biochemistry, College of Oriental Medicine and Research Inst. of Oriental Medicine and Dept. of Biomaterial Control, Graduate School and Blue-Bio Industry Regional Innovation Center, Dongeui Univ., Busan, Republic of Korea. Direct inquiries to author Choi (E-mail: choiyh@deu.ac.kr).
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  • Yung Hyun Choi

    1. Authors Park and N.D. Kim are with Dept. of Pharmacy, Pusan Natl. Univ., Busan, Republic of Korea. Author G.Y. Kim is with Dept. of Marine Life Sciences, Jeju Natl. Univ., Jeju, Republic of Korea. Author Nam is with Faculty of Food Science and Biotechnology, Pukyong Natl. Univ., Busan, Republic of Korea. Author Choi is with Dept. of Biochemistry, College of Oriental Medicine and Research Inst. of Oriental Medicine and Dept. of Biomaterial Control, Graduate School and Blue-Bio Industry Regional Innovation Center, Dongeui Univ., Busan, Republic of Korea. Direct inquiries to author Choi (E-mail: choiyh@deu.ac.kr).
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Abstract

Abstract:  Fucoidan, a sulfated polysaccharide purified from brown algae, possesses a variety of pharmacologic effects, including antiinflammatory, antioxidant, and anticancer properties; however, the underlying action mechanisms are not completely understood. This study investigated the possible mechanisms through which fucoidan exerts its antiproliferative action in cultured AGS human gastric adenocarcinoma cells. We found that fucoidan effectively inhibits the growth of AGS cells by inducing autophagy, as well as apoptosis. Apoptosis by fucoidan treatment was associated with the downregulation of antiapoptotic Bcl-2 and Bcl-xL expression, loss of mitochondrial membrane potential, activation of caspases, and concomitant degradation of poly-(ADP-ribose) polymerase protein. In addition, the morphological study indicated a characteristic finding of autophagy, such as the formation of autophagosomes in fucoidan-treated AGS cells. Furthermore, markers of autophagy, namely, the conversion of microtubule-associated protein light chain 3 (LC3)-I to LC3-II and increased beclin-1 accumulation, were observed. Overall, the present data suggest that fucoidan induces apoptotic and autophagic cell death, and both apoptotic and autophagic mechanisms contribute to the fucoidan-induced AGS cell death.

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