Antioxidant Activity and Protective Effect Against Plasmid DNA Strand Scission of Leaf, Bark, and Heartwood Extracts from Acacia catechu
Article first published online: 1 AUG 2011
© 2011 Institute of Food Technologists®
Journal of Food Science
Volume 76, Issue 7, pages C959–C964, September 2011
How to Cite
Guleria, S., Tiku, A.K., Singh, G., Vyas, D. and Bhardwaj, A. (2011), Antioxidant Activity and Protective Effect Against Plasmid DNA Strand Scission of Leaf, Bark, and Heartwood Extracts from Acacia catechu. Journal of Food Science, 76: C959–C964. doi: 10.1111/j.1750-3841.2011.02284.x
- Issue published online: 20 SEP 2011
- Article first published online: 1 AUG 2011
- MS 20101378 Submitted 12/5/2010, Accepted 5/18/2011.
- Acacia catechu;
- antioxidant activity;
- DNA nicking assay;
- free radical;
Abstract: The antioxidant activity of methanol extract/fractions of leaf, bark, and heartwood of Acacia catechu was evaluated by various antioxidant assays, including free radical, superoxide and hydroxyl radical, reducing power, metal ion chelation, as well as hydroxyl radical induced DNA strand scission. The leaf, bark, and heartwood powder was extracted in methanol and the lyophilized methanol extract was fractionated with different solvents in the order of increasing polarity. The results indicate that ethyl acetate fraction of heartwood has the highest antioxidant capacities, presenting lower EC50 values particularly in free radical scavenging activity, including DPPH radicals (4.76 ± 0.14 μg/mL), superoxide anions (26.21 ± 0.79 μg/mL), and hydroxyl radicals (33.69 ± 1.42 μg/mL), in direct assay systems. Reducing power was also highest in ethyl acetate fraction of heartwood (EC50 of 79.05 ± 1.02 μg/mL). As for the chelating power on ferrous ions, leaf extract was more effective than bark and heartwood extracts. Furthermore, the ethyl acetate and acetone fractions of heartwood significantly protected pBR322 supercoiled plasmid DNA against strand scission induced by hydroxyl radicals in a Fenton's reaction mixture.
Practical Application: The present investigation suggests that the three organs of A. catechu differ significantly in their antioxidant potential as seen in the DPPH radical scavenging assay, reducing power assay, metal ion chelating assay, superoxide radical scavenging assay and hydroxyl radical scavenging assay. Further, our results showed that crude methanol extract and ethyl acetate fraction of heartwood of A. catechu might have a good potential as a source for natural health products due to its antioxidant and DNA protective activities.