Antioxidant Capacities and Total Polyphenol Contents of Hydro-ethanolic Extract of Phytococktail from Trans-Himalaya
Version of Record online: 6 JAN 2012
© 2012 DRDO Journal of Food Science © 2012 Institute of Food Technologists®
Journal of Food Science
Volume 77, Issue 2, pages C156–C161, February 2012
How to Cite
Dhar, P., Tayade, A.B., Bajpai, P.K., Sharma, V.K., Das, S.K., Chaurasia, O.P., Srivastava, R.B. and Singh, S.B. (2012), Antioxidant Capacities and Total Polyphenol Contents of Hydro-ethanolic Extract of Phytococktail from Trans-Himalaya. Journal of Food Science, 77: C156–C161. doi: 10.1111/j.1750-3841.2011.02523.x
- Issue online: 17 FEB 2012
- Version of Record online: 6 JAN 2012
- MS 20110694 Submitted 6/2/2011, Accepted 10/17/2011.
- antioxidant capacity;
- hydro-ethanolic extract;
- sea buckthorn;
- total polyphenol content
Abstract: The aim of this study was to evaluate the in vitro antioxidant potential of hydro-ethanolic extract of a novel phytococktail comprising of sea buckthorn, apricot, and Rhodiola (SAR) from trans-Himalaya. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) activity of the extract increased in a dose-dependent manner (upto 0.1 mg/mL), and was found to be about 38% of that of ascorbic acid at 0.1 mg/mL. The hydro-ethanolic extract of SAR also scavenged the ABTS.+ radical generated by ABTS/potassium persulfate (PPS) system and was found to be about 62% of that of ascorbic acid at 0.1 mg/ mL. The total antioxidant power of the extract was determined by ferric reducing antioxidant power (FRAP) assay. Total phenolic content was found to be 1.28016 × 10−3 mol gallic acid equivalent (GAE)/g extract. Total flavonoid and flavonol contents were estimated to be 2.5970 × 10−4 mol and 4.87 × 10−4 mol quercetin equivalent/g extract, respectively. The hydro-ethanolic extract of this phytococktail indicated presence of essential phytoconstituents of polyphenols, flavonoids, flavonols, and ascorbic acid, which contributed significantly to its antioxidant capacity. The combination of the 3 plants may well support their use in traditional medicine to combat oxidative stress and high-altitude sickness.