Enhanced Antimicrobial Activity of Nisin-Loaded Liposomal Nanoparticles against Foodborne Pathogens

  1. Yunyun Zou,
  2. Hyeon-Yong Lee,
  3. Yong-Chang Seo,
  4. Juhee Ahn

Article first published online: 13 FEB 2012

DOI: 10.1111/j.1750-3841.2011.02580.x

Issue

Journal of Food Science

Journal of Food Science

Volume 77, Issue 3, pages M165–M170, March 2012

Additional Information

How to Cite

Zou, Y., Lee, H.-Y., Seo, Y.-C. and Ahn, J. (2012), Enhanced Antimicrobial Activity of Nisin-Loaded Liposomal Nanoparticles against Foodborne Pathogens. Journal of Food Science, 77: M165–M170. doi: 10.1111/j.1750-3841.2011.02580.x

Author Information

  1. Authors Zou, Lee, and Ahn are with Div. of Biomaterials Engineering, Kangwon Natl. Univ., Chuncheon, Gangwon 200-701, Republic of Korea. Author Seo is with Medical & Bio-material Research Center, Kangwon Natl. Univ., Chuncheon, Gangwon 200-701, Republic of Korea. Author Ahn is also with Inst. of Bioscience and Biotechnology, Kangwon Natl. Univ., Chuncheon, Gangwon 200-701, Republic of Korea. Direct inquiries to author Ahn (E-mail: juheeahn@kangwon.ac.kr).

Publication History

  1. Issue published online: 2 MAR 2012
  2. Article first published online: 13 FEB 2012
  3. MS 20110853 Submitted 7/15/2011, Accepted 11/28/2011.

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Keywords:

  • antimicrobial;
  • foodborne pathogen;
  • liposome;
  • nanoparticles;
  • nisin

Abstract:  This study was designed to evaluate the prolonged antimicrobial stability of nisin-loaded liposome (LipoN) nanoparticles against Listeria monocytogenes and Staphylococcus aureus. The sizes of bare liposomes and LipoN were uniformly distributed between 114 and 125 nm. The nanoparticles were homogeneously dispersed in water with less than 0.2 of polydispersity index. The zeta potential value of LipoN was +17.1 mV due to the positive charged nisin, attaining 70% of loading efficiency. The minimum inhibitory concentration of LipoN against L. monocytogenes and S. aureus was 320 international unit/mL. The LipoN significantly enhanced the antimicrobial stability in brain heart infusion agar compared to free nisin. The numbers of L. monocytogenes and S. aureus exposed to LipoN were effectively reduced by more than 6 log colony-forming unit/mL after 48 and 72 h of incubation, respectively. These results provide useful information for the development of antimicrobial delivery system to improve food safety.

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