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Antagonistic Effect of the Ainu-Selected Traditional Beneficial Plants on the Transformation of an Aryl Hydrocarbon Receptor

Authors

  • Shin Nishiumi,

    1. Author Nishiumi is with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; and Division of Gastroenterology, Dept. of Internal Medicine, Kobe Univ. Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan. Author Hosokawa is with Dept. of Nutrition Management, Faculty of Health Science, Hyogo Univ., 2310, Shinzaike, Hiraoka-cho, Kakogawa, Hyogo 675-0195, Japan. Author Anetai is with Hokkaido Inst. of Public Health Medicinal Resources Division, Faculty of Food and Pharmacy, Hokkaido Inst. of Public Health, Kita 19-Nishi 12, Kita-ku, Sapporo, Hokkaido 060-0819, Japan. Author Shibata is with Hokkaido Experimental Station for Medicinal Plants, National Inst. of Health Sciences, 108 Ohashi, Nayoro, Hokkaido 096-0065, Japan. Authors Mukai, Yoshida, and Ashida are with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan. Direct inquiries to author Ashida (E-mail: ashida@kobe-u.ac.jp).
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  • Keizo Hosokawa,

    1. Author Nishiumi is with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; and Division of Gastroenterology, Dept. of Internal Medicine, Kobe Univ. Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan. Author Hosokawa is with Dept. of Nutrition Management, Faculty of Health Science, Hyogo Univ., 2310, Shinzaike, Hiraoka-cho, Kakogawa, Hyogo 675-0195, Japan. Author Anetai is with Hokkaido Inst. of Public Health Medicinal Resources Division, Faculty of Food and Pharmacy, Hokkaido Inst. of Public Health, Kita 19-Nishi 12, Kita-ku, Sapporo, Hokkaido 060-0819, Japan. Author Shibata is with Hokkaido Experimental Station for Medicinal Plants, National Inst. of Health Sciences, 108 Ohashi, Nayoro, Hokkaido 096-0065, Japan. Authors Mukai, Yoshida, and Ashida are with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan. Direct inquiries to author Ashida (E-mail: ashida@kobe-u.ac.jp).
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  • Masaki Anetai,

    1. Author Nishiumi is with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; and Division of Gastroenterology, Dept. of Internal Medicine, Kobe Univ. Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan. Author Hosokawa is with Dept. of Nutrition Management, Faculty of Health Science, Hyogo Univ., 2310, Shinzaike, Hiraoka-cho, Kakogawa, Hyogo 675-0195, Japan. Author Anetai is with Hokkaido Inst. of Public Health Medicinal Resources Division, Faculty of Food and Pharmacy, Hokkaido Inst. of Public Health, Kita 19-Nishi 12, Kita-ku, Sapporo, Hokkaido 060-0819, Japan. Author Shibata is with Hokkaido Experimental Station for Medicinal Plants, National Inst. of Health Sciences, 108 Ohashi, Nayoro, Hokkaido 096-0065, Japan. Authors Mukai, Yoshida, and Ashida are with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan. Direct inquiries to author Ashida (E-mail: ashida@kobe-u.ac.jp).
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  • Toshiro Shibata,

    1. Author Nishiumi is with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; and Division of Gastroenterology, Dept. of Internal Medicine, Kobe Univ. Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan. Author Hosokawa is with Dept. of Nutrition Management, Faculty of Health Science, Hyogo Univ., 2310, Shinzaike, Hiraoka-cho, Kakogawa, Hyogo 675-0195, Japan. Author Anetai is with Hokkaido Inst. of Public Health Medicinal Resources Division, Faculty of Food and Pharmacy, Hokkaido Inst. of Public Health, Kita 19-Nishi 12, Kita-ku, Sapporo, Hokkaido 060-0819, Japan. Author Shibata is with Hokkaido Experimental Station for Medicinal Plants, National Inst. of Health Sciences, 108 Ohashi, Nayoro, Hokkaido 096-0065, Japan. Authors Mukai, Yoshida, and Ashida are with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan. Direct inquiries to author Ashida (E-mail: ashida@kobe-u.ac.jp).
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  • Rie Mukai,

    1. Author Nishiumi is with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; and Division of Gastroenterology, Dept. of Internal Medicine, Kobe Univ. Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan. Author Hosokawa is with Dept. of Nutrition Management, Faculty of Health Science, Hyogo Univ., 2310, Shinzaike, Hiraoka-cho, Kakogawa, Hyogo 675-0195, Japan. Author Anetai is with Hokkaido Inst. of Public Health Medicinal Resources Division, Faculty of Food and Pharmacy, Hokkaido Inst. of Public Health, Kita 19-Nishi 12, Kita-ku, Sapporo, Hokkaido 060-0819, Japan. Author Shibata is with Hokkaido Experimental Station for Medicinal Plants, National Inst. of Health Sciences, 108 Ohashi, Nayoro, Hokkaido 096-0065, Japan. Authors Mukai, Yoshida, and Ashida are with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan. Direct inquiries to author Ashida (E-mail: ashida@kobe-u.ac.jp).
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  • Ken-ichi Yoshida,

    1. Author Nishiumi is with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; and Division of Gastroenterology, Dept. of Internal Medicine, Kobe Univ. Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan. Author Hosokawa is with Dept. of Nutrition Management, Faculty of Health Science, Hyogo Univ., 2310, Shinzaike, Hiraoka-cho, Kakogawa, Hyogo 675-0195, Japan. Author Anetai is with Hokkaido Inst. of Public Health Medicinal Resources Division, Faculty of Food and Pharmacy, Hokkaido Inst. of Public Health, Kita 19-Nishi 12, Kita-ku, Sapporo, Hokkaido 060-0819, Japan. Author Shibata is with Hokkaido Experimental Station for Medicinal Plants, National Inst. of Health Sciences, 108 Ohashi, Nayoro, Hokkaido 096-0065, Japan. Authors Mukai, Yoshida, and Ashida are with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan. Direct inquiries to author Ashida (E-mail: ashida@kobe-u.ac.jp).
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  • Hitoshi Ashida

    1. Author Nishiumi is with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; and Division of Gastroenterology, Dept. of Internal Medicine, Kobe Univ. Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan. Author Hosokawa is with Dept. of Nutrition Management, Faculty of Health Science, Hyogo Univ., 2310, Shinzaike, Hiraoka-cho, Kakogawa, Hyogo 675-0195, Japan. Author Anetai is with Hokkaido Inst. of Public Health Medicinal Resources Division, Faculty of Food and Pharmacy, Hokkaido Inst. of Public Health, Kita 19-Nishi 12, Kita-ku, Sapporo, Hokkaido 060-0819, Japan. Author Shibata is with Hokkaido Experimental Station for Medicinal Plants, National Inst. of Health Sciences, 108 Ohashi, Nayoro, Hokkaido 096-0065, Japan. Authors Mukai, Yoshida, and Ashida are with Dept. of Agrobioscience, Graduate School of Agricultural Science, Kobe Univ., 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan. Direct inquiries to author Ashida (E-mail: ashida@kobe-u.ac.jp).
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Abstract

Abstract:  Transformation of an aryl hydrocarbon receptor (AhR) is the initial step to express the multiple toxicity of halogenated and polycyclic aromatic hydrocarbons (HAHs and PAHs) including dioxins. Therefore, it has been suggested that suppression of the transformation induced by HAHs and PAHs leads to reduce their toxicological effects. In this study, the antagonistic effect of 110 indigenous plants (192 plant parts) used as medicine and/or food by the Ainu on the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced AhR transformation was investigated. Of these, a stalk of Aralia elata (Miq.) Seemann and a bark of Fraxinus mandshurica Rupr. var. japonica Maxim. exhibited the strong antagonistic effect in a dose-dependent manner. An antioxidative activity and polyphenol content were also measured, and the strong correlation (r= 0.96) between these two parameters could be confirmed. However, correlation coefficients of the antagonistic effect of 192 extracts compared to their antioxidative activity and polyphenol content were 0.17 and 0.20, respectively. These results suggest that the Ainu-selected traditional beneficial plants are useful source for findings of novel AhR antagonists, and the antagonistic activity of these plants may be independent on their antioxidative activity and polyphenol content.

Practical Application:  Our findings lead to discovery of the valuable plants used by the Ainu and the novel active compounds useful for human's life, and furthermore, may contribute to the development of new medicines and functional foods.

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