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Improving Surface Functional Properties of Tofu Whey-Derived Peptides by Chemical Modification with Fatty Acids

Authors

  • Athanasia Oswald Matemu,

    1. Authors Matemu, Katayama, and Nakamura are with the Dept. of Bioscience and Biotechnology, Shinshu Univ., 8304 Minamiminowamura, Nagano 399-4598, Japan. Authors Kayahara and Murasawa are with Food Research Laboratory, Asahimatsu Foods Co. Ltd., Japan. Direct inquiries to author Nakamura (E-mail: snakamu@shinshu-u.ac.jp).
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  • Shigeru Katayama,

    1. Authors Matemu, Katayama, and Nakamura are with the Dept. of Bioscience and Biotechnology, Shinshu Univ., 8304 Minamiminowamura, Nagano 399-4598, Japan. Authors Kayahara and Murasawa are with Food Research Laboratory, Asahimatsu Foods Co. Ltd., Japan. Direct inquiries to author Nakamura (E-mail: snakamu@shinshu-u.ac.jp).
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  • Hisataka Kayahara,

    1. Authors Matemu, Katayama, and Nakamura are with the Dept. of Bioscience and Biotechnology, Shinshu Univ., 8304 Minamiminowamura, Nagano 399-4598, Japan. Authors Kayahara and Murasawa are with Food Research Laboratory, Asahimatsu Foods Co. Ltd., Japan. Direct inquiries to author Nakamura (E-mail: snakamu@shinshu-u.ac.jp).
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  • Hisashi Murasawa,

    1. Authors Matemu, Katayama, and Nakamura are with the Dept. of Bioscience and Biotechnology, Shinshu Univ., 8304 Minamiminowamura, Nagano 399-4598, Japan. Authors Kayahara and Murasawa are with Food Research Laboratory, Asahimatsu Foods Co. Ltd., Japan. Direct inquiries to author Nakamura (E-mail: snakamu@shinshu-u.ac.jp).
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  • Soichiro Nakamura

    1. Authors Matemu, Katayama, and Nakamura are with the Dept. of Bioscience and Biotechnology, Shinshu Univ., 8304 Minamiminowamura, Nagano 399-4598, Japan. Authors Kayahara and Murasawa are with Food Research Laboratory, Asahimatsu Foods Co. Ltd., Japan. Direct inquiries to author Nakamura (E-mail: snakamu@shinshu-u.ac.jp).
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Abstract

Abstract:  Effect of acylation with saturated fatty acids on surface functional properties of tofu whey-derived peptides was investigated. Tofu whey (TW) and soy proteins (7S, 11S, and acid-precipitated soy protein [APP]) were hydrolyzed by Protease M ‘Amano’ G, and resulting peptide mixtures were acylated with esterified fatty acids of different chain length (6C to 18C) to form a covalent linkage between the carboxyl group of fatty acid and the free amino groups of peptide. Acylation significantly (P < 0.05) increased emulsifying properties of 7S, 11S, and APP peptides independent of fatty acid chain length. Acylation decreased water binding capacity although oil binding capacity of acylated tofu whey ultra filtered fraction (UFTW < 3 kDa), 7S- and 11S-peptides were improved compared to native peptides. 7S peptides acylated with long chain fatty acids had shown significant higher surface hydrophobicity as in contrast with acylated UFTW < 3 kDa and APP peptides. Fluorescence spectra studies revealed structural conformation of acylated soy peptides as compared to native peptides. This study shows that chemical modification with fatty acids can further affect functional properties of soy proteins.

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