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Rapid Tomato Volatile Profiling by Using Proton-Transfer Reaction Mass Spectrometry (PTR-MS)

Authors

  • Brian Farneti,

    1. Author Farneti is with Horticultural Supply Chains Group, Wageningen Univ., Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands. Authors Cristescu and Harren are with Life Science Trace Gas Facility, Dept. of Molecular and Laser Physics, Inst. for Molecules and Materials, Radboud Univ., P.O. Box 9010, 6500 GL Nijmegen, The Netherlands. Author Costa is with Dept. of Fruit Tree and Woody Plant Science, Bologna Univ., Via Fanin 46, 40127 Bologna, Italy. Author Woltering is with Horticultural Supply Chains Group, Wageningen Univ., Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands; and Food & Biobased Research, Wageningen Univ. and Research Centre, P.O. Box 17, 6700 AA Wageningen, The Netherlands. Direct inquiries to author Farneti (E-mail: brian.farneti@gmail.com).
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  • Simona M. Cristescu,

    1. Author Farneti is with Horticultural Supply Chains Group, Wageningen Univ., Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands. Authors Cristescu and Harren are with Life Science Trace Gas Facility, Dept. of Molecular and Laser Physics, Inst. for Molecules and Materials, Radboud Univ., P.O. Box 9010, 6500 GL Nijmegen, The Netherlands. Author Costa is with Dept. of Fruit Tree and Woody Plant Science, Bologna Univ., Via Fanin 46, 40127 Bologna, Italy. Author Woltering is with Horticultural Supply Chains Group, Wageningen Univ., Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands; and Food & Biobased Research, Wageningen Univ. and Research Centre, P.O. Box 17, 6700 AA Wageningen, The Netherlands. Direct inquiries to author Farneti (E-mail: brian.farneti@gmail.com).
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  • Guglielmo Costa,

    1. Author Farneti is with Horticultural Supply Chains Group, Wageningen Univ., Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands. Authors Cristescu and Harren are with Life Science Trace Gas Facility, Dept. of Molecular and Laser Physics, Inst. for Molecules and Materials, Radboud Univ., P.O. Box 9010, 6500 GL Nijmegen, The Netherlands. Author Costa is with Dept. of Fruit Tree and Woody Plant Science, Bologna Univ., Via Fanin 46, 40127 Bologna, Italy. Author Woltering is with Horticultural Supply Chains Group, Wageningen Univ., Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands; and Food & Biobased Research, Wageningen Univ. and Research Centre, P.O. Box 17, 6700 AA Wageningen, The Netherlands. Direct inquiries to author Farneti (E-mail: brian.farneti@gmail.com).
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  • Frans J.M. Harren,

    1. Author Farneti is with Horticultural Supply Chains Group, Wageningen Univ., Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands. Authors Cristescu and Harren are with Life Science Trace Gas Facility, Dept. of Molecular and Laser Physics, Inst. for Molecules and Materials, Radboud Univ., P.O. Box 9010, 6500 GL Nijmegen, The Netherlands. Author Costa is with Dept. of Fruit Tree and Woody Plant Science, Bologna Univ., Via Fanin 46, 40127 Bologna, Italy. Author Woltering is with Horticultural Supply Chains Group, Wageningen Univ., Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands; and Food & Biobased Research, Wageningen Univ. and Research Centre, P.O. Box 17, 6700 AA Wageningen, The Netherlands. Direct inquiries to author Farneti (E-mail: brian.farneti@gmail.com).
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  • Ernst J. Woltering

    1. Author Farneti is with Horticultural Supply Chains Group, Wageningen Univ., Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands. Authors Cristescu and Harren are with Life Science Trace Gas Facility, Dept. of Molecular and Laser Physics, Inst. for Molecules and Materials, Radboud Univ., P.O. Box 9010, 6500 GL Nijmegen, The Netherlands. Author Costa is with Dept. of Fruit Tree and Woody Plant Science, Bologna Univ., Via Fanin 46, 40127 Bologna, Italy. Author Woltering is with Horticultural Supply Chains Group, Wageningen Univ., Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands; and Food & Biobased Research, Wageningen Univ. and Research Centre, P.O. Box 17, 6700 AA Wageningen, The Netherlands. Direct inquiries to author Farneti (E-mail: brian.farneti@gmail.com).
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Abstract

Abstract:  The availability of rapid and accurate methods to assess fruit flavor is of utmost importance to support quality control especially in the breeding phase. Breeders need more information and analytical tools to facilitate selection for complex multigenic traits such as flavor quality. In this study, it is shown that proton-transfer reaction mass spectrometry (PTR-MS) is a suitable method to monitor at high sensitivity the emission of volatiles determining the tomato aromatic profile such as hexanal, hexenals, methanol, ethanol, and acetaldehyde. The volatiles emitted by 14 tomato varieties (at red stage) were analyzed by 2 solvent-free headspace methods: solid-phase microextraction/gas chromatography MS and PTR-MS. Multivariate statistics (principal component analysis and cluster analysis) of the PTR-MS results allow an unambiguous separation between varieties, especially with a clear fingerprinting separation between the different tomato types: round truss, cocktail, and cherry tomatoes. PTR-MS was also successfully used to monitor the changes in volatile profiles during postharvest ripening and storage.

Practical Application:  These results show that proton-transfer reaction mass spectrometry (PTR-MS) is suited to monitor at high sensitivity the emission of a large number of volatiles that describe the tomato aroma profile. This technology can easily monitor and quantify compounds related to ripening and/or senescence so that it can be used to improve the breeding of new fruit and vegetable cultivars based on volatiles. Moreover, PTR-MS can be used to monitor changes in volatile profile during ripening and storage.

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