Chemical Composition of Currant Seed Extracts and Their Protective Effect on Human Lymphocytes DNA

Authors

  • Dejan Gođevac,

    1. Authors Gođevac and Vajs are with Inst. of Chemistry, Technology and Metallurgy, Njegoševa 12, Univ. of Belgrade, Serbia. Authors Tešević and Milosavljević are with Faculty of Chemistry, Studentski trg 12-16, Univ. of Belgrade, Serbia. Author Zdunić is with Inst. for Medicinal Plants Research Dr Josif Pančić, Tadeuša Košćuška 1, Belgrade, Serbia. Author Đorđević is with Faculty of Agriculture, Nemanjina 6, Zemun, Univ. of Belgrade, Serbia. Author Stanković is with Inst. of Nuclear Science ″Vinča″, Univ. of Belgrade, Serbia. Direct inquiries to author Gođevac (E-mail: dgodjev@chem.bg.ac.rs).
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  • Vele Tešević,

    1. Authors Gođevac and Vajs are with Inst. of Chemistry, Technology and Metallurgy, Njegoševa 12, Univ. of Belgrade, Serbia. Authors Tešević and Milosavljević are with Faculty of Chemistry, Studentski trg 12-16, Univ. of Belgrade, Serbia. Author Zdunić is with Inst. for Medicinal Plants Research Dr Josif Pančić, Tadeuša Košćuška 1, Belgrade, Serbia. Author Đorđević is with Faculty of Agriculture, Nemanjina 6, Zemun, Univ. of Belgrade, Serbia. Author Stanković is with Inst. of Nuclear Science ″Vinča″, Univ. of Belgrade, Serbia. Direct inquiries to author Gođevac (E-mail: dgodjev@chem.bg.ac.rs).
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  • Vlatka Vajs,

    1. Authors Gođevac and Vajs are with Inst. of Chemistry, Technology and Metallurgy, Njegoševa 12, Univ. of Belgrade, Serbia. Authors Tešević and Milosavljević are with Faculty of Chemistry, Studentski trg 12-16, Univ. of Belgrade, Serbia. Author Zdunić is with Inst. for Medicinal Plants Research Dr Josif Pančić, Tadeuša Košćuška 1, Belgrade, Serbia. Author Đorđević is with Faculty of Agriculture, Nemanjina 6, Zemun, Univ. of Belgrade, Serbia. Author Stanković is with Inst. of Nuclear Science ″Vinča″, Univ. of Belgrade, Serbia. Direct inquiries to author Gođevac (E-mail: dgodjev@chem.bg.ac.rs).
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  • Slobodan Milosavljević,

    1. Authors Gođevac and Vajs are with Inst. of Chemistry, Technology and Metallurgy, Njegoševa 12, Univ. of Belgrade, Serbia. Authors Tešević and Milosavljević are with Faculty of Chemistry, Studentski trg 12-16, Univ. of Belgrade, Serbia. Author Zdunić is with Inst. for Medicinal Plants Research Dr Josif Pančić, Tadeuša Košćuška 1, Belgrade, Serbia. Author Đorđević is with Faculty of Agriculture, Nemanjina 6, Zemun, Univ. of Belgrade, Serbia. Author Stanković is with Inst. of Nuclear Science ″Vinča″, Univ. of Belgrade, Serbia. Direct inquiries to author Gođevac (E-mail: dgodjev@chem.bg.ac.rs).
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  • Gordana Zdunić,

    1. Authors Gođevac and Vajs are with Inst. of Chemistry, Technology and Metallurgy, Njegoševa 12, Univ. of Belgrade, Serbia. Authors Tešević and Milosavljević are with Faculty of Chemistry, Studentski trg 12-16, Univ. of Belgrade, Serbia. Author Zdunić is with Inst. for Medicinal Plants Research Dr Josif Pančić, Tadeuša Košćuška 1, Belgrade, Serbia. Author Đorđević is with Faculty of Agriculture, Nemanjina 6, Zemun, Univ. of Belgrade, Serbia. Author Stanković is with Inst. of Nuclear Science ″Vinča″, Univ. of Belgrade, Serbia. Direct inquiries to author Gođevac (E-mail: dgodjev@chem.bg.ac.rs).
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  • Boban ĐOrđević,

    1. Authors Gođevac and Vajs are with Inst. of Chemistry, Technology and Metallurgy, Njegoševa 12, Univ. of Belgrade, Serbia. Authors Tešević and Milosavljević are with Faculty of Chemistry, Studentski trg 12-16, Univ. of Belgrade, Serbia. Author Zdunić is with Inst. for Medicinal Plants Research Dr Josif Pančić, Tadeuša Košćuška 1, Belgrade, Serbia. Author Đorđević is with Faculty of Agriculture, Nemanjina 6, Zemun, Univ. of Belgrade, Serbia. Author Stanković is with Inst. of Nuclear Science ″Vinča″, Univ. of Belgrade, Serbia. Direct inquiries to author Gođevac (E-mail: dgodjev@chem.bg.ac.rs).
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  • Miroslava Stanković

    1. Authors Gođevac and Vajs are with Inst. of Chemistry, Technology and Metallurgy, Njegoševa 12, Univ. of Belgrade, Serbia. Authors Tešević and Milosavljević are with Faculty of Chemistry, Studentski trg 12-16, Univ. of Belgrade, Serbia. Author Zdunić is with Inst. for Medicinal Plants Research Dr Josif Pančić, Tadeuša Košćuška 1, Belgrade, Serbia. Author Đorđević is with Faculty of Agriculture, Nemanjina 6, Zemun, Univ. of Belgrade, Serbia. Author Stanković is with Inst. of Nuclear Science ″Vinča″, Univ. of Belgrade, Serbia. Direct inquiries to author Gođevac (E-mail: dgodjev@chem.bg.ac.rs).
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Abstract

Abstract:  On the basis of LC/UV/MS analysis, 35 compounds from the extracts of seeds of black, red, and white currants (2 cultivars of each) were identified. Black currants cultivars contained protocatechuic acid and p-hydroxybenzoic acid, and traces of nitril containing phenolic acids. The presence of synapoyl glucoside was characteristic for cv. Malling Jewel. Sesquiterpenoid glycosides and carboxymethylindol glycosides were present mainly in white and red currant cultivars. Blackcurrant seeds contained higher amounts of flavonoids, especially rutin, isoquercetin, and taxifolin. The currant seed extracts were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. The frequency of MN was scored in binucleated cells, and the cultivars of black currants showed the best antioxidant potential. At a concentration of only 0.17 mg/mL, extract of the seed cv. Malling Jewel effected a decrease in the frequency of MN of 60% compared with control cell cultures.

Practical Application:  Our results provide evidence of protective effects of currant seed extracts and isolated pure compounds on cytogenetic damages in human lymphocytes. Thus, currant seed extracts could exert beneficial effects in quite a few diseases, for many of the biological actions have been attributed to their antioxidant properties.

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