Dr. E. Allen Foegeding served as Scientific Editor and Dr. Shridhar Sathe served as Associate Editor overseeing single-blinded review of this manuscript. It is the policy of JFS to blind Editorial Board members from the peer-review process of their own submissions, just as all authors are blinded.
Nitrite-Cured Color and Phosphate-Mediated Water Binding of Pork Muscle Proteins as Affected by Calcium in the Curing Solution
Article first published online: 3 JUL 2012
© 2012 Institute of Food Technologists®
Journal of Food Science
Volume 77, Issue 7, pages C811–C817, July 2012
How to Cite
Zhao, J. and Xiong, Y. L. (2012), Nitrite-Cured Color and Phosphate-Mediated Water Binding of Pork Muscle Proteins as Affected by Calcium in the Curing Solution. Journal of Food Science, 77: C811–C817. doi: 10.1111/j.1750-3841.2012.02766.x
- Issue published online: 3 JUL 2012
- Article first published online: 3 JUL 2012
- MS 20120188 Submitted 2/7/2012, Accepted 4/23/2012.
- meat curing;
- protein extract color;
- water-holding capacity
Abstract: Calcium is a mineral naturally present in water and may be included into meat products during processing thereby influencing meat quality. Phosphates improve myofibril swelling and meat water-holding capacity (WHC) but can be sensitive to calcium precipitation. In this study, pork shoulder meat was used to investigate the impact of calcium at 0, 250, and 500 ppm and phosphate type [sodium pyrophosphate (PP), tripolyphosphate (TPP), and hexametaphopshate (HMP)] at 10 mM on nitrite-cured protein extract color at various pH levels (5.5, 6.0, and 6.5) and crude myofibril WHC at pH 6.0. Neither calcium nor phosphates present in the curing brines significantly affected the cured color. Increasing the pH tended to promote the formation of metmyoglobin instead of nitrosylmyoglobin. The ability of PP to enhance myofibril WHC was hampered (P < 0.05) by increasing the calcium concentration due to PP precipitation. Calcium also decreased the solubility of TPP but did not influence its enhancement of WHC. On the other hand, HMP was more tolerant of calcium but the soluble Ca-HMP complex was less effective than free HMP to promote water binding by myofibrils. The depressed muscle fiber swelling responding to added calcium as evidenced by phase contrast microscopy substantiated, to a certain extent, the deleterious effect of calcium, suggesting that hardness of curing water can significantly affect the quality of cured meat products.
Practical Application: Although not affecting nitrite-cured color, calcium hampers the efficacy of phosphates to promote water binding by muscle proteins, underscoring the importance of water quality for brine-enhanced meat products.