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Efficacy of Sardinelle Protein Hydrolysate to Alleviate Ethanol-Induced Oxidative Stress in the Heart of Adult Rats

Authors

  • Zeineb Kamoun,

    1. Authors Kamoun, Chtourou, and Zeghal are with Animal Physiology Laboratory, Life Sciences Dept., Faculty of Sciences, BP 1171, 3000 Sfax, Univ. of Sfax, Tunisia. Authors Kamoun, Bougatef, and Nasri are with Enzymatic Engineering and Microbiology Laboratory (LGEM), Engineer National School, BP 1173, 3000 Sfax, Univ. of Sfax, Tunisia. Author Boudawara is with Anatomopathology Laboratory, CHU Habib Bourguiba, 3029 Sfax, Univ. of Sfax, Tunisia. Direct inquiries to author Zeghal (E-mail: Naj_Zgh@yahoo.fr).
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  • Alya Sellami Kamoun,

    1. Authors Kamoun, Chtourou, and Zeghal are with Animal Physiology Laboratory, Life Sciences Dept., Faculty of Sciences, BP 1171, 3000 Sfax, Univ. of Sfax, Tunisia. Authors Kamoun, Bougatef, and Nasri are with Enzymatic Engineering and Microbiology Laboratory (LGEM), Engineer National School, BP 1173, 3000 Sfax, Univ. of Sfax, Tunisia. Author Boudawara is with Anatomopathology Laboratory, CHU Habib Bourguiba, 3029 Sfax, Univ. of Sfax, Tunisia. Direct inquiries to author Zeghal (E-mail: Naj_Zgh@yahoo.fr).
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  • Ali Bougatef,

    1. Authors Kamoun, Chtourou, and Zeghal are with Animal Physiology Laboratory, Life Sciences Dept., Faculty of Sciences, BP 1171, 3000 Sfax, Univ. of Sfax, Tunisia. Authors Kamoun, Bougatef, and Nasri are with Enzymatic Engineering and Microbiology Laboratory (LGEM), Engineer National School, BP 1173, 3000 Sfax, Univ. of Sfax, Tunisia. Author Boudawara is with Anatomopathology Laboratory, CHU Habib Bourguiba, 3029 Sfax, Univ. of Sfax, Tunisia. Direct inquiries to author Zeghal (E-mail: Naj_Zgh@yahoo.fr).
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  • Yassine Chtourou,

    1. Authors Kamoun, Chtourou, and Zeghal are with Animal Physiology Laboratory, Life Sciences Dept., Faculty of Sciences, BP 1171, 3000 Sfax, Univ. of Sfax, Tunisia. Authors Kamoun, Bougatef, and Nasri are with Enzymatic Engineering and Microbiology Laboratory (LGEM), Engineer National School, BP 1173, 3000 Sfax, Univ. of Sfax, Tunisia. Author Boudawara is with Anatomopathology Laboratory, CHU Habib Bourguiba, 3029 Sfax, Univ. of Sfax, Tunisia. Direct inquiries to author Zeghal (E-mail: Naj_Zgh@yahoo.fr).
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  • Tahia Boudawara,

    1. Authors Kamoun, Chtourou, and Zeghal are with Animal Physiology Laboratory, Life Sciences Dept., Faculty of Sciences, BP 1171, 3000 Sfax, Univ. of Sfax, Tunisia. Authors Kamoun, Bougatef, and Nasri are with Enzymatic Engineering and Microbiology Laboratory (LGEM), Engineer National School, BP 1173, 3000 Sfax, Univ. of Sfax, Tunisia. Author Boudawara is with Anatomopathology Laboratory, CHU Habib Bourguiba, 3029 Sfax, Univ. of Sfax, Tunisia. Direct inquiries to author Zeghal (E-mail: Naj_Zgh@yahoo.fr).
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  • Moncef Nasri,

    1. Authors Kamoun, Chtourou, and Zeghal are with Animal Physiology Laboratory, Life Sciences Dept., Faculty of Sciences, BP 1171, 3000 Sfax, Univ. of Sfax, Tunisia. Authors Kamoun, Bougatef, and Nasri are with Enzymatic Engineering and Microbiology Laboratory (LGEM), Engineer National School, BP 1173, 3000 Sfax, Univ. of Sfax, Tunisia. Author Boudawara is with Anatomopathology Laboratory, CHU Habib Bourguiba, 3029 Sfax, Univ. of Sfax, Tunisia. Direct inquiries to author Zeghal (E-mail: Naj_Zgh@yahoo.fr).
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  • Najiba Zeghal

    1. Authors Kamoun, Chtourou, and Zeghal are with Animal Physiology Laboratory, Life Sciences Dept., Faculty of Sciences, BP 1171, 3000 Sfax, Univ. of Sfax, Tunisia. Authors Kamoun, Bougatef, and Nasri are with Enzymatic Engineering and Microbiology Laboratory (LGEM), Engineer National School, BP 1173, 3000 Sfax, Univ. of Sfax, Tunisia. Author Boudawara is with Anatomopathology Laboratory, CHU Habib Bourguiba, 3029 Sfax, Univ. of Sfax, Tunisia. Direct inquiries to author Zeghal (E-mail: Naj_Zgh@yahoo.fr).
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Abstract

Abstract:  The present study was undertaken to examine the protective effects of sardinelle proteins hydrolysate (SPH) obtained from heads and viscera against ethanol toxicity in the heart of adult rats. Twenty-four male rats of Wistar strain, weighing at the beginning of the experiment 250 to 300 g, were used in this study. They were divided into 4 groups: group (C) served as controls, group (Eth) received 30% ethanol solution at 3 g/kg body weight, group (SPH) received only 7.27 mg of SPH/kg body weight, and group (Eth-SPH) received ethanol and sardinelle proteins hydrolysate simultaneously. All treatments were made by gavage during 15 d. Treatment with ethanol revealed a significant elevation of malondialdehyde and protein carbonyl levels in the heart and of aspartate transaminase and alanine transaminase activities in plasma. Nitric oxide levels and the activities of antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase decreased. Nonenzymatic antioxidant such as reduced glutathione did not significantly change and ascorbic acid was decreased. SPH intake concomitantly with ethanol restored these parameters to near control values. These modifications confirmed histopathological aspects of the heart. The results revealed that SPH could provide protection of the myocardium against ethanol-induced oxidative damages in rats. This may be due to the high antioxidant potential of SPH.

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