Radical Scavenging Activity and LC-MS Metabolic Profiling of Petals, Stamens, and Flowers of Crocus sativus L.
Article first published online: 18 JUL 2012
© 2012 Institute of Food Technologists®
Journal of Food Science
Volume 77, Issue 8, pages C893–C900, August 2012
How to Cite
Montoro, P., Maldini, M., Luciani, L., Tuberoso, C. I. G., Congiu, F. and Pizza, C. (2012), Radical Scavenging Activity and LC-MS Metabolic Profiling of Petals, Stamens, and Flowers of Crocus sativus L. Journal of Food Science, 77: C893–C900. doi: 10.1111/j.1750-3841.2012.02803.x
- Issue published online: 3 AUG 2012
- Article first published online: 18 JUL 2012
- MS 20120289 Submitted 2/24/2012, Accepted 5/2/2012.
- Crocus sativus;
- Free radical scavenging activity
Abstract: Radical scavenging activities of Crocus sativus petals, stamens and entire flowers, which are waste products in the production of the spice saffron, by employing ABTS radical scavenging method, were determined. At the same time, the metabolic profiles of different extract (obtained by petals, stamens and flowers) were obtained by LC-ESI-IT MS (liquid chromatography coupled with electrospray mass spectrometry equipped with Ion Trap analyser). LC-ESI-MS is a techniques largely used nowadays for qualitative fingerprint of herbal extracts and particularly for phenolic compounds. To compare the different extracts under an analytical point of view a specific method for qualitative LC-MS analysis was developed. The high variety of glycosylated flavonoids found in the metabolic profiles could give value to C. sativus petals, stamens and entire flowers.
Practical Application: Waste products obtained during saffron production, could represent an interesting source of phenolic compounds, with respect to the high variety of compounds and their free radical scavenging activity.